In vitro comparison of conventional hyperthermia and modulated electro-hyperthermia

Radiofrequency-induced hyperthermia (HT) treatments for cancer include conventional capacitive coupling hyperthermia (cCHT) and modulated electro-hyperthermia (mEHT). In this study, we directly compared these methods with regard to in vitro cytotoxicity and mechanisms of action under isothermal cond...

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Published in:Oncotarget 2016-12, Vol.7 (51), p.84082-84092
Main Authors: Yang, Kai-Lin, Huang, Cheng-Chung, Chi, Mau-Shin, Chiang, Hsin-Chien, Wang, Yu-Shan, Hsia, Chien-Chung, Andocs, Gabor, Wang, Hsin-Ell, Chi, Kwan-Hwa
Format: Article
Language:English
Subjects:
Apoptosis
beta Catenin - metabolism
Cadherins - metabolism
Calreticulin - metabolism
Caspases - metabolism
Cell Line, Tumor
Cell Membrane - metabolism
Hep G2 Cells
Hot Temperature
HSP70 Heat-Shock Proteins - metabolism
Humans
Hyperthermia, Induced - methods
MCF-7 Cells
Neoplasms - metabolism
Neoplasms - pathology
Reactive Oxygen Species - metabolism
Research Paper
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Summary:Radiofrequency-induced hyperthermia (HT) treatments for cancer include conventional capacitive coupling hyperthermia (cCHT) and modulated electro-hyperthermia (mEHT). In this study, we directly compared these methods with regard to in vitro cytotoxicity and mechanisms of action under isothermal conditions. Hepatoma (HepG2) cells were exposed to HT treatment (42°C for 30 min) using mEHT, cCHT or a water bath. mEHT produced a much higher apoptosis rate (43.1% ± 5.8%) than cCHT (10.0% ± 0.6%), the water bath (8.4% ± 1.7%) or a 37°C control (6.6% ± 1.1%). The apoptosis-inducing effect of mEHT at 42°C was similar to that achieved with a water bath at 46°C. mEHT also increased expression of caspase-3, 8 and 9. All three hyperthermia methods increased intracellular heat shock protein 70 (Hsp70) levels, but only mEHT greatly increased the release of Hsp70 from cells. Calreticulin and E-cadherin levels in the cell membrane also increased after mEHT treatment, but not after cCHT or water bath. These results suggest that mEHT selectively deposits energy on the cell membrane and may be a useful treatment modality that targets cancer cell membranes.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.11444