Influence of Mechanical Force on Bone Matrix Proteins in Ovariectomised Mice and Osteoblast-like MC3T3-E1 Cells

To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells. Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill train...

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Bibliographic Details
Published in:In vivo (Athens) 2017-01, Vol.31 (1), p.87-96
Main Authors: Zhang, Meng, Ishikawa, Shintaro, Inagawa, Tomoko, Ikemoto, Hideshi, Guo, Shiyu, Sunagawa, Masataka, Hisamitsu, Tadashi
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion Molecules - pharmacology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix Proteins - genetics
Extracellular Matrix Proteins - metabolism
Female
Gene Expression Regulation - drug effects
Humans
Immunoenzyme Techniques
Mice
Mice, Inbred ICR
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoporosis, Postmenopausal - drug therapy
Osteoporosis, Postmenopausal - etiology
Osteoporosis, Postmenopausal - metabolism
Ovariectomy
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Semaphorin-3A - genetics
Semaphorin-3A - metabolism
Stress, Mechanical
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Summary:To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells. Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro. In OVX+Run group tibias, the number of tartrate-resistant acid phosphatase-positive osteoclasts was lower than in the OVX group, and the expression of periostin and semaphorin-3A was higher. In MC3T3-E1 cells, centrifugal stress significantly increased periostin and semaphorin-3A mRNA expression. Treatment with periostin increased the semaphorin-3A level. We speculate that mechanical load may increase periostin production in osteoblasts, and periostin may inhibit osteoclast differentiation by its effects on semaphorin-3A. Our results support the concept of a positive correlation between exercise and inhibition of osteoclasts in post-menopausal osteoporosis.
ISSN:1791-7549
0258-851X
1791-7549
DOI:10.21873/invivo.11029