A quick and robust MHC typing method for free-ranging and captive primate species

Gene products of the major histocompatibility complex (MHC) of human and non-human primates play a crucial role in adaptive immunity, and most of the relevant genes not only show a high degree of variability (polymorphism) but also copy number variation (CNV) is observed. Due to this diversity, MHC...

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Bibliographic Details
Published in:Immunogenetics (New York) 2017-04, Vol.69 (4), p.231-240
Main Authors: de Groot, N., Stanbury, K., de Vos-Rouweler, A. J. M., de Groot, N. G., Poirier, N., Blancho, G., de Luna, C., Doxiadis, G. G. M., Bontrop, R. E.
Format: Article
Language:English
Subjects:
Allergology
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Conservation biology
DNA Copy Number Variations - genetics
Endangered & extinct species
Female
Gene Function
Genes
Genotype
Haplotypes
Human Genetics
Human health and pathology
Humans
Hylobates moloch
Immunology
Life Sciences
Macaca
Major Histocompatibility Complex - genetics
Male
Microsatellite Repeats - genetics
Monkeys & apes
Original
Original Article
Papio anubis
Pathogens
Phylogeny
Polymorphism
Polymorphism, Genetic - genetics
Primates
Primates - classification
Primates - genetics
Wildlife conservation
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Summary:Gene products of the major histocompatibility complex (MHC) of human and non-human primates play a crucial role in adaptive immunity, and most of the relevant genes not only show a high degree of variability (polymorphism) but also copy number variation (CNV) is observed. Due to this diversity, MHC proteins influence the capability of individuals to cope with various pathogens. MHC and/or MHC-linked gene products such as odorant receptor genes are thought to influence mate choice and reproductive success. Therefore, MHC typing of wild and captive primate populations is considered to be useful in conservation biology, which is, however, often hampered by the need of invasive and time-consuming methods. All intact Mhc-DRB genes in primates appear to possess a complex and highly divergent microsatellite, DRB-STR. A panel of 154 pedigreed olive baboons ( Papio anubis ) was examined for their DRB content by DRB-STR analysis of genomic DNA. Using the same methodology on DNA of feces samples, DRB variability of a silvery gibbon population ( Hylobates moloch ) ( N  = 24), an endangered species, could successfully be studied. In both species, length determination of the DRB-STR resulted in the definition of unique genotyping patterns that appeared to be specific for a certain chromosome. Moreover, the different STR lengths were shown to segregate with the allelic variation of the respective gene. The results obtained expand data gained previously on DRB-STR typing in macaques, great apes, and humans and strengthen the conclusion that this protocol is applicable in molecular ecology, conservation biology, and colony management, especially of endangered primate species.
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-016-0968-0