Aurora B expression modulates paclitaxel response in non-small cell lung cancer

Taxanes are mitotic poisons widely used in the treatment of non-small cell lung cancer (NSCLC), however, little is known about potential molecular modulators of response to these compounds. Aurora B (AURKB) is a critical regulator of the mitotic spindle assembly, previously shown overexpressed in NS...

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Bibliographic Details
Published in:British journal of cancer 2017-02, Vol.116 (5), p.592-599
Main Authors: Al-Khafaji, Ahmed Sk, Davies, Michael Pa, Risk, Janet M, Marcus, Michael W, Koffa, Maria, Gosney, John R, Shaw, Richard J, Field, John K, Liloglou, Triantafillos
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Antineoplastic Agents, Phytogenic - therapeutic use
Aurora Kinase B - genetics
Carcinoma, Non-Small-Cell Lung - drug therapy
Carcinoma, Non-Small-Cell Lung - genetics
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic - drug effects
Humans
Lung cancer
Lung Neoplasms - drug therapy
Lung Neoplasms - genetics
Male
Middle Aged
Organophosphates - pharmacology
Paclitaxel - therapeutic use
Quinazolines - pharmacology
Translational Therapeutics
Up-Regulation - drug effects
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Summary:Taxanes are mitotic poisons widely used in the treatment of non-small cell lung cancer (NSCLC), however, little is known about potential molecular modulators of response to these compounds. Aurora B (AURKB) is a critical regulator of the mitotic spindle assembly, previously shown overexpressed in NSCLC. Here we investigated the hypothesis that AURKB expression modulates the efficacy of taxanes in NSCLC cells. AURKB mRNA expression was determined by qPCR in 132 frozen NSCLC tissues and nine NSCLC cell lines. Aurora B expression was knocked down in cell lines using multiple shRNA constructs. Barasertib was used to specifically inhibit AURKB activity, determined by the level of H3S10 phosphorylation. Frequent AURKB mRNA upregulation was observed in NSCLC tissues (P
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.2016.453