NF-κB signaling regulates cell-autonomous regulation of CXCL10 in breast cancer 4T1 cells

The chemokine CXCL10 and its receptor CXCR3 play a role in breast cancer metastasis to bone and osteoclast activation. However, the mechanism of CXCL10/CXCR3-induced intracellular signaling has not been fully investigated. To evaluate CXCL10-induced cellular events in the mouse breast cancer cell li...

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Published in:Experimental & molecular medicine 2017-02, Vol.49 (2), p.e295-e295
Main Authors: Jin, Won Jong, Kim, Bongjun, Kim, Darong, Park Choo, Hea-Young, Kim, Hong-Hee, Ha, Hyunil, Lee, Zang Hee
Format: Article
Language:English
Subjects:
Animals
Breast - immunology
Breast - pathology
Breast Neoplasms - immunology
Breast Neoplasms - pathology
Cell Line, Tumor
Chemokine CXCL10 - analysis
Chemokine CXCL10 - immunology
Female
Mice
Mice, Inbred BALB C
NF-kappa B - analysis
NF-kappa B - immunology
Original
Receptors, CXCR3 - antagonists & inhibitors
Receptors, CXCR3 - immunology
Signal Transduction
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Summary:The chemokine CXCL10 and its receptor CXCR3 play a role in breast cancer metastasis to bone and osteoclast activation. However, the mechanism of CXCL10/CXCR3-induced intracellular signaling has not been fully investigated. To evaluate CXCL10-induced cellular events in the mouse breast cancer cell line 4T1, we developed a new synthetic CXCR3 antagonist JN-2. In this study, we observed that secretion of CXCL10 in the supernatant of 4T1 cells was gradually increased during cell growth. JN-2 inhibited basal and CXCL10-induced CXCL10 expression and cell motility in 4T1 cells. Treatment of 4T1 cells with CXCL10 increased the expression of P65, a subunit of the NF-κB pathway, via activation of the NF-κB transcriptional activity. Ectopic overexpression of P65 increased CXCL10 secretion and blunted JN-2-induced suppression of CXCL10 secretion, whereas overexpression of IκBα suppressed CXCL10 secretion. These results indicate that the CXCL10/CXCR3 axis creates a positive feedback loop through the canonical NF-κB signaling pathway in 4T1 cells. In addition, treatment of osteoblasts with conditioned medium from JN-2-treated 4T1 cells inhibited the expression of RANKL, a crucial cytokine for osteoclast differentiation, which resulted in an inhibitory effect on osteoclast differentiation in the co-culture system of bone marrow-derived macrophages and osteoblasts. Direct intrafemoral injection of 4T1 cells induced severe bone destruction; however, this effect was suppressed by the CXCR3 antagonist via downregulation of P65 expression in an animal model. Collectively, these results suggest that the CXCL10/CXCR3-mediated NF-κB signaling pathway plays a role in the control of autonomous regulation of CXCL10 and malignant tumor properties in breast cancer 4T1 cells.
ISSN:2092-6413
1226-3613
2092-6413
DOI:10.1038/emm.2016.148