Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae

Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characte...

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Published in:Veterinary research (Paris) 2017-02, Vol.48 (1), p.4-4, Article 4
Main Authors: Sassu, Elena L, Ladinig, Andrea, Talker, Stephanie C, Stadler, Maria, Knecht, Christian, Stein, Heiko, Frömbling, Janna, Richter, Barbara, Spergser, Joachim, Ehling-Schulz, Monika, Graage, Robert, Hennig-Pauka, Isabel, Gerner, Wilhelm
Format: Article
Language:English
Subjects:
Actinobacillus
Actinobacillus Infections - immunology
Actinobacillus Infections - microbiology
Actinobacillus Infections - pathology
Actinobacillus Infections - veterinary
Actinobacillus pleuropneumoniae - immunology
Animals
Causes of
Chronic Disease
Health aspects
Identification and classification
Immune response
Life Sciences
Lung - immunology
Lung - microbiology
Lung - pathology
Lung diseases
Lymph Nodes - pathology
Male
Observations
Pleuropneumonia - immunology
Pleuropneumonia - microbiology
Pleuropneumonia - pathology
Pleuropneumonia - veterinary
Swine
Swine Diseases - immunology
Swine Diseases - microbiology
Swine Diseases - pathology
T cells
Th17 Cells - pathology
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Summary:Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characterized. To address this, pigs were intranasally infected with APP serotype 2 and euthanized during the acute phase [6-10 days post-infection (dpi)] or the chronic phase of APP infection (27-31 dpi). Lymphocytes isolated from blood, tonsils, lung tissue and tracheobronchial lymph nodes were analyzed by intracellular cytokine staining (ICS) for IL-17A, IL-10 and TNF-α production after in vitro stimulation with crude capsular extract (CCE) of the APP inoculation strain. This was combined with cell surface staining for the expression of CD4, CD8α and TCR-γδ. Clinical records, microbiological investigations and pathological findings confirmed the induction of a subclinical APP infection. ICS-assays revealed the presence of APP-CCE specific CD4 CD8α IL-17A-producing T cells in blood and lung tissue in most infected animals during the acute and chronic phase of infection and a minor fraction of these cells co-produced TNF-α. APP-CCE specific IL-17A-producing γδ T cells could not be found and APP-CCE specific IL-10-producing CD4 T cells were present in various organs but only in a few infected animals. The frequency of identified putative Th17 cells (CD4 CD8α IL-17A ) in lung and blood correlated positively with lung lesion scores and APP-specific antibody titers during the chronic phase. These results suggest a potential role of Th17 cells in the immune pathogenesis of APP infection.
ISSN:1297-9716
0928-4249
1297-9716
DOI:10.1186/s13567-017-0411-z