Comparison of rumen bacteria distribution in original rumen digesta, rumen liquid and solid fractions in lactating Holstein cows

Background： Original rumen digesta, rumen liquid and solid fractions have been frequently used to assess the rumen bacterial community. However, bacterial profiles in rumen original digesta, liquid and solid fractions vary from each other and need to be better established.Methods： To compare bacteri...

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Bibliographic Details
Published in:Journal of animal science and biotechnology 2017-02, Vol.8 (3), p.636-642, Article 16
Main Authors: Ji, Shoukun, Zhang, Hongtao, Yan, Hui, Azarfar, Arash, Shi, Haitao, Alugongo, Gibson, Li, Shengli, Cao, Zhijun, Wang, Yajing
Format: Article
Language:English
Subjects:
Bacteria
Bar codes
Diet
Genetic testing
Software
固体
奶牛
液体
生物标志物
瘤胃内容物
瘤胃细菌
组分
细菌多样性
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Summary:Background： Original rumen digesta, rumen liquid and solid fractions have been frequently used to assess the rumen bacterial community. However, bacterial profiles in rumen original digesta, liquid and solid fractions vary from each other and need to be better established.Methods： To compare bacterial profiles in each fraction, samples of rumen digesta from six cows fed either a high fiber diet（HFD） or a high energy diet（HED） were collected via rumen fistulas. Rumen digesta was then squeezed through four layers of cheesecloth to separate liquid and solid fractions. The bacterial profiles of rumen original digesta, liquid and solid fractions were analyzed with High-throughput sequencing technique.Results： Rumen bacterial diversity was mainly affected by diet and individual cow（P 〉 0.05） rather than rumen fraction. Bias distributed bacteria were observed in solid and liquid fractions of rumen content using Venn diagram and LEf Se analysis. Fifteen out of 16 detected biomarkers（using LEf Se analysis） were found in liquid fraction, and these 15 biomarkers contributed the most to the bacterial differences among rumen content fractions.Conclusions： Similar results were found when using samples of original rumen digesta, rumen liquid or solid fractions to assess diversity of rumen bacteria; however, more attention should be draw onto bias distributed bacteria in different ruminal fractions, especially when liquid fraction has been used as a representative sample for rumen bacterial study.
ISSN:1674-9782
2049-1891
2049-1891
DOI:10.1186/s40104-017-0142-z