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Low Incidence along with Low mRNA Levels of EGFRvIII in Prostate and Colorectal Cancers Compared to Glioblastoma
Background: The presence as well as the potential role of EGFR vIII in tumors other than glioblastoma still remains a controversial subject with many contradictory data published. Previous analyses, however, did not consider the level of EGFR vIII mRNA expression in different tumor types. Methods: A...
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Published in: | Journal of Cancer 2017-01, Vol.8 (1), p.146-151 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Background:
The presence as well as the potential role of EGFR
vIII
in tumors other than glioblastoma still remains a controversial subject with many contradictory data published. Previous analyses, however, did not consider the level of
EGFR
vIII
mRNA expression in different tumor types.
Methods:
Appropriately designed protocol for Real-time quantitative reverse-transcription PCR (Real-time qRT-PCR) was applied to analyze
EGFR
vIII
and
EGFR
WT
mRNA expression in 155 tumor specimens. Additionally, Western Blot (WB) analysis was performed for selected samples. Stable cell lines showing EGFR
vIII
expression (CAS-1 and DK-MG) were analyzed by means of WB, immunocytochemistry (ICC) and fluorescence
in situ
hybridization (FISH).
Results:
Our analyses revealed
EGFR
vIII
expression in 27.59% of glioblastomas (8/29), 8.11% of colorectal cancers (3/37), 6.52% of prostate cancers (3/46) and none of breast cancers (0/43). Despite the average relative expression of
EGFR
vIII
varying greatly among tumors of different tissues (approximately 800-fold) or even within the same tissue group (up to 8000-fold for GB), even the marginal expression of
EGFR
vIII
mRNA can be detrimental to cancer progression, as determined by the analysis of stable cell lines endogenously expressing the oncogene.
Conclusion:
EGFR
vIII
plays an unquestionable role in glioblastomas with high expression of this oncogene. Our data suggests that EGFR
vIII
importance should not be underestimated even in tumors with relatively low expression of this oncogene. |
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ISSN: | 1837-9664 1837-9664 |
DOI: | 10.7150/jca.16108 |