Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum

Background： The chicken gastrointestinal tract contains a diverse microbiota whose composition and structure play important roles in gut functionality. In this study, microbial shifts resulting from feed supplementation with Bacillus subtilis CSL2 were evaluated in broilers challenged and unchalleng...

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Bibliographic Details
Published in:Journal of animal science and biotechnology 2017-01, Vol.8 (2), p.450-458, Article 1
Main Authors: Oh, Ju Kyoung, Pajarillo, Edward Alain B, Chae, Jong Pyo, Kim, In Ho, Yang, Dong Soo, Kang, Dae-Kyung
Format: Article
Language:English
Subjects:
Animals
Bacillus
Diet
Discriminant analysis
Feeds
Homeostasis
Infections
Microbiota
Microorganisms
Pathogens
Phylogenetics
Poultry
Probiotics
Salmonella
Software
Taxonomy
Titanium
伤寒沙门氏菌
功能多样性
微生物生态学
枯草芽孢杆菌
粪便菌群
组成
肉鸡日粮
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Summary:Background： The chicken gastrointestinal tract contains a diverse microbiota whose composition and structure play important roles in gut functionality. In this study, microbial shifts resulting from feed supplementation with Bacillus subtilis CSL2 were evaluated in broilers challenged and unchallenged with Salmonella Gallinarum. To analyse bacterial community composition and functionality, 454 GS-FLX pyrosequencing of 16 S r RNA gene amplicons was performed.Results： The Quantitative Insights into Microbial Ecology（QIIME） pipeline was used to analyse changes in the faecal microbiota over a 24-h period. A total of 718,204 sequences from broiler chickens were recorded and analysed. At the phylum level, Firmicutes, Bacteroidetes, and Proteobacteria were the predominant bacterial taxa. In Salmonellainfected chickens（SC）, Bacteroidetes were more highly abundant compared to control（NC） and Bacillus-treated（BT）chickens. At the genus level, in the NC and BT groups, Lactobacil us was present at high abundance, and the abundance of Turicibacter, unclassified Enterobacteriaceae, and Bacteroides increased in SC broilers. Furthermore, taxon-independent analysis showed that the SC and BT groups were compositional y distinct at the end of the 24-h period. Further analysis of functional properties showed that B. subtilis CSL2 administration increased gut-associated energy supply mechanisms（i.e. carbohydrate transport and metabolism） to maintain a stable microbiota and protect gut integrity.Conclusions： This study demonstrated that S. Gallinarum infection and B. subtilis CSL2 supplementation in the diet of broiler chickens influenced the diversity, composition, and functional diversity of the faecal microbiota. Moreover, the findings offer significant insights to understand potential mechanisms of Salmonel a infection and the mode of action of probiotics in broiler chickens.
ISSN:1674-9782
2049-1891
2049-1891
DOI:10.1186/s40104-016-0130-8