Loading…
The LBD12-1 Transcription Factor Suppresses Apical Meristem Size by Repressing Argonaute 10 Expression1
Rice LBD12-1 functions as a repressor of shoot apical meristem size by down-regulating expression of AGO10 and HD-ZIP III. The shoot apical meristem ( SAM ) consists of a population of multipotent cells that generates all aerial structures and regenerates itself. SAM maintenance and lateral organ de...
Saved in:
Published in: | Plant physiology (Bethesda) 2016-11, Vol.173 (1), p.801-811 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Rice LBD12-1 functions as a repressor of shoot apical meristem size by down-regulating expression of AGO10 and HD-ZIP III.
The shoot apical meristem (
SAM
) consists of a population of multipotent cells that generates all aerial structures and regenerates itself.
SAM
maintenance and lateral organ development are regulated by several complex signaling pathways, in which the
Argonaute
gene-mediated pathway plays a key role. One
Argonaute
gene,
AGO10
, functions as a microRNA locker that attenuates miR165/166 activity and positively regulates shoot apical meristem development, but little is known about when and how
AGO10
is regulated at the transcriptional level. In this work, we showed that transgenic rice plants overexpressing
LBD12-1
, an
LBD
family transcription factor, exhibited stunted growth, twisted leaves, abnormal anthers, and reduced SAM size. Further research revealed that LBD12-1 directly binds to the promoter region and represses the expression of
AGO10.
Overexpression of
AGO10
in an
LBD12-1
overexpression background rescued the growth defect phenotype of
LBD12-1
-overexpressing plants. The expression of
LBD12-1
and its binding ability to the
AGO10
promoter is induced by stress.
lbd12-1
loss-of-function mutants showed similar phenotypes and SAM size to the wild type under normal conditions, but
lbd12-1
had a larger SAM under salt stress. Our findings provide novel insights into the regulatory mechanism of AGO10 by which SAM size is controlled under stress conditions. |
---|---|
ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.16.01699 |