Posttranslational modifications of apolipoprotein A-II proteoforms in type 2 diabetes

Background Apolipoprotein A-II (apoA-II) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. Objective Our objective was to characterize plasma apoA-II proteoforms in pa...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical lipidology 2016-07, Vol.10 (4), p.808-815
Main Authors: Azizkhanian, Ida, MSc, Trenchevska, Olgica, PhD, Bashawri, Yara, MPH, Hu, Jiaqi, MPH, Koska, Juraj, MD, PhD, Reaven, Peter D., MD, Nelson, Randall W., PhD, Nedelkov, Dobrin, PhD, Yassine, Hussein N., MD
Format: Article
Language:English
Subjects:
Adult
Apolipoprotein A-II
Apolipoprotein A-II - blood
Apolipoprotein A-II - metabolism
Cardiovascular
Case-Control Studies
Diabetes
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - metabolism
Female
Humans
Male
Mass spectrometry
Oxidations
Protein Processing, Post-Translational
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Apolipoprotein A-II (apoA-II) is the second most abundant protein in high-density lipoprotein particles. However, it exists in plasma in multiple forms. The effect of diabetes on apoA-II proteoforms is not known. Objective Our objective was to characterize plasma apoA-II proteoforms in participants with and without type 2 diabetes. Methods Using a novel mass spectrometric immunoassay, the relative abundance of apoA-II proteoforms was examined in plasma of 30 participants with type 2 diabetes and 25 participants without diabetes. Results Six apoA-II proteoforms (monomer, truncated TQ monomer, truncated Q monomer, dimer, truncated Q dimer, and truncated 2Qs dimer) and their oxidized proteoforms were identified. The ratios of oxidized monomer and all oxidized proteoforms to the native apoA-II were significantly greater in the diabetic group ( P  = .004 and P  = .005, respectively) compared with the nondiabetic group. Conclusion The relative abundance of oxidized apoA-II is significantly increased in type 2 diabetes.
ISSN:1933-2874
1876-4789
DOI:10.1016/j.jacl.2016.03.001