Epithelial ovarian cancer-secreted exosomal miR-222-3p induces polarization of tumor-associated macrophages

Cancer secreted exosomal miRNAs are emerging as mediators between tumor-stoma crosstalk. Here, we show epithelial ovarian cancer (EOC)-derived exosomes activated macrophages to a tumor-associated macrophage (TAM)-like phenotype with SOCS3/STAT3 pathway involvement, which could facilitate the progres...

Full description

Saved in:
Bibliographic Details
Published in:Oncotarget 2016-07, Vol.7 (28), p.43076-43087
Main Authors: Ying, Xiang, Wu, Quanfeng, Wu, Xiaoli, Zhu, Qinyi, Wang, Xinjing, Jiang, Lu, Chen, Xin, Wang, Xipeng
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - genetics
Carcinoma, Ovarian Epithelial
Cell Line, Tumor
Down-Regulation
Exosomes - genetics
Female
Gene Expression Profiling - methods
Humans
Macrophage Activation - genetics
Macrophages - metabolism
Mice, Nude
MicroRNAs - genetics
Neoplasms, Glandular and Epithelial - blood
Neoplasms, Glandular and Epithelial - genetics
Ovarian Neoplasms - blood
Ovarian Neoplasms - genetics
Research Paper
STAT3 Transcription Factor - genetics
Suppressor of Cytokine Signaling 3 Protein - genetics
Transplantation, Heterologous
U937 Cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cancer secreted exosomal miRNAs are emerging as mediators between tumor-stoma crosstalk. Here, we show epithelial ovarian cancer (EOC)-derived exosomes activated macrophages to a tumor-associated macrophage (TAM)-like phenotype with SOCS3/STAT3 pathway involvement, which could facilitate the progression of cancer. MiR-222-3p was enrichment in exosomes released from EOC cells and it could be transferred to macrophages. Overexpression of miR-222-3p in macrophages induced polarization of the M2 phenotype. Luciferase assay verified miR-222-3p targeted SOCS3 genes and expression of SOCS3 was decreased after transfection with a miR-222-3p mimic. Down-regulation of SOCS3 correlated with an increased expression of STAT3 activation. MiR-222-3p could be detected in the exosomes from serum and its levels were related to EOC. These observations propose tumor-derived exosomal miR-222-3p is an effective regulator in the polarization of tumor-promoting M2 macrophages and may be a biomarker of EOC.
ISSN:1949-2553
1949-2553
DOI:10.18632/oncotarget.9246