Molecular Cloning and Functional Analysis of a Na + -Insensitive K + Transporter of Capsicum chinense Jacq

High-affinity K (HAK) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. These HAK-type transporters participate in low- and high-affinity potassium (K ) uptake and are crucial for the maintenance of K homeostasis under hostile conditions. In this study, the...

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Published in:Frontiers in plant science 2016-12, Vol.7, p.1980-1980
Main Authors: Ruiz-Lau, Nancy, Bojórquez-Quintal, Emanuel, Benito, Begoña, Echevarría-Machado, Ileana, Sánchez-Cach, Lucila A, Medina-Lara, María de Fátima, Martínez-Estévez, Manuel
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Language:English
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Plant Science
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Summary:High-affinity K (HAK) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. These HAK-type transporters participate in low- and high-affinity potassium (K ) uptake and are crucial for the maintenance of K homeostasis under hostile conditions. In this study, the full-length cDNA of gene was isolated from roots of the habanero pepper ( ). expression was positively regulated by K starvation in roots and was not inhibited in the presence of NaCl. Phylogenetic analysis placed the CcHAK1 transporter in group I of the HAK K transporters, showing that it is closely related to CaHAK1 and LeHAK5. Characterization of the protein in a yeast mutant deficient in high-affinity K uptake (WΔ3) suggested that CcHAK1 function is associated with high-affinity K uptake, with K and V for Rb of 50 μM and 0.52 nmol mg min , respectively. K uptake in yeast expressing the CcHAK1 transporter was inhibited by millimolar concentrations of the cations ammonium ([Formula: see text]) and cesium (Cs ) but not by sodium (Na ). The results presented in this study suggest that the CcHAK1 transporter may contribute to the maintenance of K homeostasis in root cells in plants undergoing K -deficiency and salt stress.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2016.01980