Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study

AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle r...

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Published in:World journal of gastroenterology : WJG 2016-12, Vol.22 (47), p.10341-10352
Main Authors: Das, Dipanwita, Sarkar, Neelakshi, Sengupta, Isha, Pal, Ananya, Saha, Debraj, Bandopadhyay, Manikankana, Das, Chandrima, Narayan, Jimmy, Singh, Shivram Prasad, Chakravarty, Runu
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Basic Study
Cellular Microenvironment
DNA Methylation
DNA, Viral - genetics
Dose-Response Relationship, Drug
Epigenesis, Genetic
G1 Phase Cell Cycle Checkpoints
Hep G2 Cells
Hepacivirus - genetics
Hepacivirus - pathogenicity
Hepatitis B, Chronic - genetics
Hepatitis B, Chronic - metabolism
Hepatitis B, Chronic - prevention & control
Hepatitis B, Chronic - virology
Hepatocytes - drug effects
Hepatocytes - metabolism
Hepatocytes - virology
Host-Pathogen Interactions
Humans
Ligands
Lipopolysaccharides - pharmacology
NF-kappa B - metabolism
Signal Transduction
Time Factors
Toll-Like Receptor 4 - agonists
Toll-Like Receptor 4 - genetics
Toll-Like Receptor 4 - metabolism
Viral Load
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Summary:AIM Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4(TLR4) in hepatitis B virus(HBV) infection.METHODS Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the Hep G2.2.15 cells with TLR4 specific ligand. Nuclear factor(NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.RESULTS The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.CONCLUSION The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v22.i47.10341