Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity

Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble S...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-11, Vol.6 (1), p.37953-37953, Article 37953
Main Authors: Pepin, Marion, Mezouar, Soraya, Pegon, Julie, Muczynski, Vincent, Adam, Frédéric, Bianchini, Elsa P, Bazaa, Amine, Proulle, Valerie, Rupin, Alain, Paysant, Jerome, Panicot-Dubois, Laurence, Christophe, Olivier D, Dubois, Christophe, Lenting, Peter J, Denis, Cécile V
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
Antigens, CD - genetics
Antigens, CD - metabolism
Antigens, CD - pharmacology
Antigens, Differentiation, Myelomonocytic - genetics
Antigens, Differentiation, Myelomonocytic - metabolism
Antigens, Differentiation, Myelomonocytic - pharmacology
Antiinflammatory agents
Calcium
CD162 antigen
Disease Models, Animal
E-Selectin - metabolism
Female
Humans
Inflammation
Inflammation - chemically induced
Inflammation - drug therapy
Inflammation - pathology
Lectins - genetics
Lectins - metabolism
Lectins - pharmacology
Leukocyte rolling
Leukocyte Rolling - physiology
Leukocytes (mononuclear)
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Life Sciences
Ligands
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Mice, Inbred C57BL
P-selectin
P-Selectin - metabolism
Perfusion
Peripheral blood mononuclear cells
Protein Interaction Domains and Motifs
Rodents
Selectins
Solubility
Tumor Necrosis Factor-alpha - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. Pre-treatment of PSGL1 with sialidase reduced Siglec-5 binding by 79 ± 4%. In confocal immune-fluorescence assays, we observed that 50% of Peripheral Blood Mononuclear Cells (PBMCs) simultaneously express PSGL1 and Siglec-5. Duolink-proximity ligation analysis demonstrated that PSGL1 and Siglec-5 are in close proximity (
ISSN:2045-2322
2045-2322
DOI:10.1038/srep37953