Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains un...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2016-11, Vol.164, p.96-102
Main Authors: Tatmatsu-Rocha, José Carlos, Ferraresi, Cleber, Hamblin, Michael R., Damasceno Maia, Flávio, do Nascimento, Nilberto Robson Falcão, Driusso, Patricia, Parizotto, Nivaldo Antonio
Format: Article
Language:English
Subjects:
Animals
Catalase
Collagen production
Diabetes mellitus
Low-Level Light Therapy
Mice
Nitric oxide
Nitrosation - radiation effects
Oxidative stress
Oxidative Stress - radiation effects
Photobiomodulation
Skin - metabolism
Skin - radiation effects
TBARS
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Summary:Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains unclear. This study aimed to investigate whether LLLT was able to improve oxidative/nitrosative stress parameters in the wound healing process in diabetic mice. Twenty male mice were divided into four groups: non-irradiated control (NIC), irradiated control (IC), non-irradiated and diabetic (NID), irradiated and diabetic (ID). Diabetes was induced by administration of streptozotocin. Wounds were created 120days after the induction of diabetes in groups IC and ID and these groups were irradiated daily for 5days (superpulsed 904nm laser, average power 40mW, 60s). All animals were sacrificed 1day after the last irradiation and histology, collagen amount, catalase activity, nitrite and thiobarbituric acid reactive substances (TBARS) were measured. Histology showed that collagen fibers were more organized in IC and ID when compared to NID group, and significant differences in collagen content were found in group ID versus NID. Catalase activity was higher in IC group compared to other groups (p
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2016.09.017