Wild-type p53 binds to MYC promoter G-quadruplex

G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathwa...

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Bibliographic Details
Published in:Bioscience reports 2016-10, Vol.36 (5), p.e00397-e00397
Main Authors: Petr, Marek, Helma, Robert, Polášková, Alena, Krejčí, Aneta, Dvořáková, Zuzana, Kejnovská, Iva, Navrátilová, Lucie, Adámik, Matej, Vorlíčková, Michaela, Brázdová, Marie
Format: Article
Language:English
Subjects:
Circular Dichroism
DNA - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
G-Quadruplexes
Gene Expression Regulation
HCT116 Cells
Humans
Original Paper
Original Papers
Promoter Regions, Genetic - genetics
Protein Binding
Proto-Oncogene Proteins c-myc - genetics
Proto-Oncogene Proteins c-myc - metabolism
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
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Summary:G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.
ISSN:0144-8463
1573-4935
DOI:10.1042/bsr20160232