Human sex hormone-binding globulin as a potential target of alternate plasticizers: an in silico study

Currently, alternate plasticizers are used to replace phthalate plasticizers in children's toys, medical equipments and food packaging, due to the adverse effects of phthalate compounds on human health and laws prohibiting their use. Current information regarding the safety and potential advers...

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Bibliographic Details
Published in:BMC structural biology 2016-09, Vol.16 (Suppl 1), p.15-15, Article 15
Main Authors: Sheikh, Ishfaq A, Yasir, Muhammad, Abu-Elmagd, Muhammad, Dar, Tanveer A, Abuzenadah, Adel M, Damanhouri, Ghazi A, Al-Qahtani, Mohammed, Beg, Mohd A
Format: Article
Language:English
Subjects:
Benzoates - chemistry
Benzoates - metabolism
Binding proteins
Chemical properties
Globulin
Health aspects
Humans
Molecular Docking Simulation
Phthalic Acids - chemistry
Phthalic Acids - metabolism
Plasticizers
Plasticizers - chemistry
Plasticizers - metabolism
Protein Binding
Sex Hormone-Binding Globulin - chemistry
Sex Hormone-Binding Globulin - metabolism
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Summary:Currently, alternate plasticizers are used to replace phthalate plasticizers in children's toys, medical equipments and food packaging, due to the adverse effects of phthalate compounds on human health and laws prohibiting their use. Current information regarding the safety and potential adverse effects of alternate plasticizers is limited and recent studies have found alternate plasticizers to display similar characteristics to those observed in phthalate plasticizers. This study was undertaken to evaluate and predict the potential endocrine disrupting activity of the three most commonly used alternate plasticizers: di(2-ethylhexyl)terephthalate (DEHT), tris(2-ethylhexyl)trimellitate (TOTM), and diisononyl hexahydrophthalate (DINCH) against human sex hormone-binding globulin (SHBG) using in silico approaches. The crystal structure of human SHBG (Id: 1D2S) was retrieved from Protein Data Bank. PubChem database was searched for the structures of alternate plasticizers, DEHT, TOTM, and DINCH. Docking was performed using Glide (Schrodinger) Induced Fit Docking module. Induced Fit Docking of three alternate plasticizer compounds indicated that each of the three compounds fitted well into the steroid binding pocket of SHBG. Docking displays showed interactions of alternate plasticizers with 25-30 amino-acid residues of SHBG; 18-20 amino residues overlapped between the natural ligand, DHT, and the three compounds (commonality of 82-91 %). The hydrogen-bonding interaction of the amino-acid residue, Asn-82, of SHBG was also present in displays of DHT and all the three alternate phthalates. The binding affinity of all the three alternate phthalates was higher than DHT; maximum in DINCH followed by TOTM and DEHT. Our results suggested that the three alternate plasticizers have potential to engage the important interacting residues of SHBG and thus interfere in its steroid homeostatic function.
ISSN:1472-6807
1472-6807
DOI:10.1186/s12900-016-0067-3