Elucidation of flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida plant extracts by hyphenated technique of liquid chromatography-mass spectroscopy

Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant sp...

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Published in:	Pharmacognosy research 2016-10, Vol.8 (4), p.281-286
Main Authors:	Doshi, Gaurav, Nalawade, Vivek, Mukadam, Aaditi, Chaskar, Pratip, Zine, Sandeep, Somani, Rakesh, Une, Hemant
Format:	Article
Language:	eng
Subjects:	Original
Online Access:	Get full text
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Summary:	Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.
ISSN:	0974-8490 0976-4836 0974-8490