The deficiency of galectin-3 in stromal cells leads to enhanced tumor growth and bone marrow metastasis

Galectin-3 is a multifunctional β-galactoside-binding lectin that once synthesized, is expressed in the nucleus, cytoplasm, cell surface and in the extracellular environment. Because of its unique structure, galectin-3 can oligomerize forming lattice upon binding to multivalent oligossacharides and...

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Published in:BMC cancer 2016-08, Vol.16 (1), p.636-636, Article 636
Main Authors: Pereira, Jonathas Xavier, Azeredo, Maria Carolina Braga, Martins, Felipe Sá, Chammas, Roger, Oliveira, Felipe Leite, Santos, Sofia Nascimento, Bernardes, Emerson Soares, El-Cheikh, Márcia Cury
Format: Article
Language:English
Subjects:
Analysis
Animals
Bone Marrow Neoplasms - genetics
Bone Marrow Neoplasms - pathology
Bone Marrow Neoplasms - secondary
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell Line, Tumor
Cell Proliferation
Female
Galectin 3 - deficiency
Galectin 3 - genetics
Immunohistochemistry
Lectins
Lymph Nodes
Metastasis
Mice, Inbred BALB C
Neoplasm Transplantation
Physiological aspects
Risk factors
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Summary:Galectin-3 is a multifunctional β-galactoside-binding lectin that once synthesized, is expressed in the nucleus, cytoplasm, cell surface and in the extracellular environment. Because of its unique structure, galectin-3 can oligomerize forming lattice upon binding to multivalent oligossacharides and influence several pathologic events such as tumorigenesis, invasion and metastasis. In our study, balb/c Lgals3+/+ and Lgals3-/- female mice were inoculated in the fourth mammary fat pad with 4T1 breast cancer cell line. The primary tumor, inguinal lymph nodes and iliac bone marrow were evaluated 15, 21 and 28 days post-injection. The primary tumor growth was evaluated by measuring the external diameter, internal growth by ultrasound and weight of the excised tumor. The presence of cancer cells in the draining lymph nodes and iliac crest bone marrow were performed by immunohistochemistry, PCR and clonogenic metastatic assay. In this study we demonstrated that the deletion of galectin-3 in the host affected drastically the in vivo growth rate of 4T1 tumors. The primary tumors in Lgals3-/- mice displayed a higher proliferative rate (p 
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-016-2679-1