Müller glia as an important source of cytokines and inflammatory factors present in the gliotic retina during proliferative vitreoretinopathy

Retinal gliosis is characterized by biochemical and physiological changes that often lead to Müller glia proliferation and hypertrophy and is a feature of many neuro‐degenerative and inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although Müller glia are known to release inflam...

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Bibliographic Details
Published in:Glia 2016-04, Vol.64 (4), p.495-506
Main Authors: Eastlake, K., Banerjee, P. J., Angbohang, A., Charteris, D. G., Khaw, P. T., Limb, G. A.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Cell Line
Cytokines
Cytokines - metabolism
Ependymoglial Cells - immunology
Humans
Immunoblotting
Middle Aged
Milk
Müller glia
PVR
Retina
Retina - immunology
Retina - surgery
retina degeneration
retinal gliosis
Side effects
Vitreoretinopathy, Proliferative - immunology
Vitreoretinopathy, Proliferative - surgery
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Summary:Retinal gliosis is characterized by biochemical and physiological changes that often lead to Müller glia proliferation and hypertrophy and is a feature of many neuro‐degenerative and inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although Müller glia are known to release inflammatory factors and cytokines, it is not clear whether cytokine production by these cells mirrors the pattern of factors present in the gliotic retina. Lysates from normal cadaveric retina and gliotic retinal specimens from patients undergoing retinectomy for treatment of PVR, the Müller cell line MIO‐M1 and four human Müller glial cell preparations isolated from normal retina were examined for their expression of cytokines and inflammatory factors using semi‐quantitative dot blot antibody arrays and quantitative arrays. Comparative analysis of the expression of inflammatory factors showed that in comparison with normal retina, gliotic retina exhibited greater than twofold increase in 24/102 factors examined by semiquantitative arrays, and a significant increase in 19 out of 27 factors assessed by quantitative methods (P 
ISSN:0894-1491
1098-1136
DOI:10.1002/glia.22942