Prospective evaluation of SeptiFast Multiplex PCR in children with systemic inflammatory response syndrome under antibiotic treatment

Antimicrobially pre-treated children with systemic inflammation often pose a diagnostic challenge to the physician. We aimed to evaluate the additional use of SeptiFast multiplex polymerase chain reaction (PCR) to identify causative pathogens in children with suspected systemic bacterial or fungal i...

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Bibliographic Details
Published in:BMC infectious diseases 2016-08, Vol.16 (1), p.378-378, Article 378
Main Authors: Gies, Franziska, Tschiedel, Eva, Felderhoff-Müser, Ursula, Rath, Peter-Michael, Steinmann, Joerg, Dohna-Schwake, Christian
Format: Article
Language:English
Subjects:
Adolescent
Analysis
Anti-Bacterial Agents - therapeutic use
Antibiotics
Area Under Curve
Aspergillus fumigatus - genetics
Bacteremia - diagnosis
Bacteremia - drug therapy
Bacteremia - metabolism
Bacteremia - microbiology
C-Reactive Protein - metabolism
Calcitonin - metabolism
Candida - genetics
Child
Child, Preschool
Children
Enterococcus - genetics
Escherichia coli - genetics
Female
Fungemia - diagnosis
Fungemia - drug therapy
Fungemia - metabolism
Fungemia - microbiology
Health aspects
Humans
Infant
Infant, Newborn
Infectious diseases
Inflammation
Klebsiella - genetics
Male
Multiplex Polymerase Chain Reaction
Mycoses
Pathogenic microorganisms
Polymerase chain reaction
Prospective Studies
Pseudomonas - genetics
Real-Time Polymerase Chain Reaction
Risk factors
Sensitivity and Specificity
Sepsis - diagnosis
Sepsis - metabolism
Sepsis - microbiology
Staphylococcus aureus - genetics
Streptococcus - genetics
Systemic Inflammatory Response Syndrome - diagnosis
Systemic Inflammatory Response Syndrome - metabolism
Systemic Inflammatory Response Syndrome - microbiology
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Summary:Antimicrobially pre-treated children with systemic inflammation often pose a diagnostic challenge to the physician. We aimed to evaluate the additional use of SeptiFast multiplex polymerase chain reaction (PCR) to identify causative pathogens in children with suspected systemic bacterial or fungal infection. Prospective observational study in 39 children with systemic inflammatory response syndrome (SIRS) under empiric antibiotic treatment. Primary outcome was the rate of positive blood cultures (BC), compared to the rate of positive SeptiFast (SF) results. In total, 14 SF-samples yielded positive results, compared to 4 positive BC (p 
ISSN:1471-2334
1471-2334
DOI:10.1186/s12879-016-1722-9