Mitofusin-2 is required for mouse oocyte meiotic maturation

Mitofusin-2 (Mfn2) is essential for embryonic development, anti-apoptotic events, protection against free radical-induced lesions, and mitochondrial fusion in many cells. However, little is known about its mechanism and function during oocyte maturation. In this study, we found that Mfn2 was express...

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Bibliographic Details
Published in:Scientific reports 2016-08, Vol.6 (1), p.30970, Article 30970
Main Authors: Zhang, Jing-Hua, Zhang, Teng, Gao, Si-Hua, Wang, Ke, Yang, Xiu-Yan, Mo, Fang-Fang, Na Yu, An, Tian, Li, Yu-Feng, Hu, Ji-Wei, Jiang, Guang-Jian
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Chromosomes
Chromosomes, Mammalian - genetics
Chromosomes, Mammalian - metabolism
Cytoplasm
Embryogenesis
Female
Gene Knockdown Techniques
GTP Phosphohydrolases - genetics
GTP Phosphohydrolases - metabolism
MAP kinase
Maturation
Meiosis
Meiosis - physiology
Metaphase
Mice
Mice, Inbred ICR
Mitochondria
Mitochondrial DNA
Oocytes
Oocytes - cytology
Oocytes - metabolism
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
siRNA
Spindles
Tubulin
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Summary:Mitofusin-2 (Mfn2) is essential for embryonic development, anti-apoptotic events, protection against free radical-induced lesions, and mitochondrial fusion in many cells. However, little is known about its mechanism and function during oocyte maturation. In this study, we found that Mfn2 was expressed in the cytoplasm during different stages of mouse oocyte maturation. Mfn2 was mainly associated with α-tubulin during oocyte maturation. Knockdown of Mfn2 by specific siRNA injection into oocytes caused the mitochondrial morphology and quantity to change, resulting in severely defective spindles and misaligned chromosomes. This led to metaphase I arrest and the failure of first polar body extrusion. Furthermore, Mfn2 depletion from GV stage oocytes caused the redistribution of p38 MAPK in oocyte cytoplasm. These findings provide insights into potential mechanisms of Mfn2-mediated cellular alterations, which may have significant implications for oocyte maturation.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep30970