Characterizing human lung tissue microbiota and its relationship to epidemiological and clinical features

The human lung tissue microbiota remains largely uncharacterized, although a number of studies based on airway samples suggest the existence of a viable human lung microbiota. Here we characterized the taxonomic and derived functional profiles of lung microbiota in 165 non-malignant lung tissue samp...

Full description

Saved in:
Bibliographic Details
Published in:Genome Biology 2016-07, Vol.17 (1), p.163-163, Article 163
Main Authors: Yu, Guoqin, Gail, Mitchell H, Consonni, Dario, Carugno, Michele, Humphrys, Michael, Pesatori, Angela C, Caporaso, Neil E, Goedert, James J, Ravel, Jacques, Landi, Maria Teresa
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biodiversity
Bioinformatics
Bronchitis
Cancer therapies
Chronic obstructive pulmonary disease
Deoxyribonucleic acid
DNA
DNA Barcoding, Taxonomic
Epidemiology
Female
Genes
Genomes
Health care
Humans
Laboratories
Lung - microbiology
Lung cancer
Lung Neoplasms - complications
Lung Neoplasms - epidemiology
Lung Neoplasms - pathology
Male
Metagenome
Metagenomics - methods
Metastases
Microbiota
Middle Aged
Neoplasm Staging
Organ Specificity
Pathogens
Phylogenetics
Population density
Reproductive Tract Infections - complications
Reproductive Tract Infections - epidemiology
Reproductive Tract Infections - microbiology
Respiratory tract
Risk Factors
RNA, Ribosomal, 16S - genetics
Skin
Studies
Taxonomy
Tobacco smoking
Tumors
Vagina
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human lung tissue microbiota remains largely uncharacterized, although a number of studies based on airway samples suggest the existence of a viable human lung microbiota. Here we characterized the taxonomic and derived functional profiles of lung microbiota in 165 non-malignant lung tissue samples from cancer patients. We show that the lung microbiota is distinct from the microbial communities in oral, nasal, stool, skin, and vagina, with Proteobacteria as the dominant phylum (60 %). Microbiota taxonomic alpha diversity increases with environmental exposures, such as air particulates, residence in low to high population density areas, and pack-years of tobacco smoking and decreases in subjects with history of chronic bronchitis. Genus Thermus is more abundant in tissue from advanced stage (IIIB, IV) patients, while Legionella is higher in patients who develop metastases. Moreover, the non-malignant lung tissues have higher microbiota alpha diversity than the paired tumors. Our results provide insights into the human lung microbiota composition and function and their link to human lifestyle and clinical outcomes. Studies among subjects without lung cancer are needed to confirm our findings.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-016-1021-1