Salmonella Degrades the Host Glycocalyx Leading to Altered Infection and Glycan Remodeling

Complex glycans cover the gut epithelial surface to protect the cell from the environment. Invasive pathogens must breach the glycan layer before initiating infection. While glycan degradation is crucial for infection, this process is inadequately understood. Salmonella contains 47 glycosyl hydrolas...

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Bibliographic Details
Published in:Scientific reports 2016-07, Vol.6 (1), p.29525-29525, Article 29525
Main Authors: Arabyan, Narine, Park, Dayoung, Foutouhi, Soraya, Weis, Allison M, Huang, Bihua C, Williams, Cynthia C, Desai, Prerak, Shah, Jigna, Jeannotte, Richard, Kong, Nguyet, Lebrilla, Carlito B, Weimer, Bart C
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Caco-2 Cells
Cell Line
Gene Deletion
Gene Expression Regulation
Glycocalyx - chemistry
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Host-Pathogen Interactions
Humans
In Vitro Techniques
Intestinal Mucosa - chemistry
Intestinal Mucosa - microbiology
Polysaccharides - chemistry
Polysaccharides - metabolism
Proteolysis
Salmonella
Salmonella typhi - enzymology
Salmonella typhi - pathogenicity
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Complex glycans cover the gut epithelial surface to protect the cell from the environment. Invasive pathogens must breach the glycan layer before initiating infection. While glycan degradation is crucial for infection, this process is inadequately understood. Salmonella contains 47 glycosyl hydrolases (GHs) that may degrade the glycan. We hypothesized that keystone genes from the entire GH complement of Salmonella are required to degrade glycans to change infection. This study determined that GHs recognize the terminal monosaccharides (N-acetylneuraminic acid (Neu5Ac), galactose, mannose, and fucose) and significantly (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/srep29525