Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro

Epigenetic changes have been recently recognized as important in human cancers. Enhancer of zeste homologue 2 gene (EZH2) has been shown the overexpression in various human cancers, consistent with a straightforward role of EZH2 as an oncogene, but its function in carcinogenesis is partly contradict...

Full description

Saved in:
Bibliographic Details
Published in:Experimental biology and medicine (Maywood, N.J.) N.J.), 2015-04, Vol.240 (4), p.458-466
Main Authors: He, Song-Bing, Zhou, Hao, Zhou, Jian, Zhou, Guo-Qiang, Han, Tuo, Wan, Dai-Wei, Gu, Wen, Gao, Lin, Zhang, Yi, Xue, Xiao-Feng, Zhang, Li-Feng, Fei, Min, Hu, Shui-Qing, Yang, Xiao-Dong, Zhu, Xin-Guo, Wang, Liang, Li, De-Chun
Format: Article
Language:English
Subjects:
Adenocarcinoma - diagnosis
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Apoptosis - drug effects
Apoptosis - physiology
Biomarkers, Tumor - metabolism
Cell Cycle - drug effects
Cell Cycle - physiology
Cell Line, Tumor
Cell Movement - drug effects
Cell Movement - physiology
Cell Proliferation - drug effects
Cell Proliferation - physiology
Colorectal Neoplasms - diagnosis
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
Enhancer of Zeste Homolog 2 Protein
Female
Humans
In Vitro Techniques
Male
Middle Aged
Original Research
Polycomb Repressive Complex 2 - antagonists & inhibitors
Polycomb Repressive Complex 2 - drug effects
Polycomb Repressive Complex 2 - metabolism
Prognosis
RNA, Small Interfering - pharmacology
Up-Regulation - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epigenetic changes have been recently recognized as important in human cancers. Enhancer of zeste homologue 2 gene (EZH2) has been shown the overexpression in various human cancers, consistent with a straightforward role of EZH2 as an oncogene, but its function in carcinogenesis is partly contradictory. The role of EZH2 in development of human colorectal cancer (CRC) has not yet been clarified. In the present study, we observed up-regulation of EZH2 expression in tumor tissues from CRC patients. The expression of EZH2 in CRC cell lines is consistent with the trend in cancer tissues using reverse transcription polymerase chain reaction (RT-PCR). We showed that TNM stage and lymph node metastasis in CRC patients are significantly correlated with EZH2 expression levels. EZH2 level of transcription and protein is inhibited by small interfering RNA (siRNA). More importantly, EZH2-siRNA inhibits the proliferation and migration of SW620 cells while promoting their apoptosis, and inducing G0/G1 cell cycle arrest of SW620 cells. Collectively, our results suggest that upregulated EZH2 expression may contribute to the progression of the patients with CRC. A comprehensive study of epigenetic mechanisms and the relevance of EZH2 in CRC is important for fully understanding this disease and as a basis for developing new treatment options in patients with CRC.
ISSN:1535-3702
1535-3699
DOI:10.1177/1535370214542215