A Subpopulation of Olfactory Bulb GABAergic Interneurons Is Derived from Emx1- and Dlx5/6-Expressing Progenitors

The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin+, calbindin+, and dopaminergic (TH+) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain...

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Bibliographic Details
Published in:The Journal of neuroscience 2007-06, Vol.27 (26), p.6878-6891
Main Authors: Kohwi, Minoree, Petryniak, Magdalena A, Long, Jason E, Ekker, Marc, Obata, Kunihiko, Yanagawa, Yuchio, Rubenstein, John L. R, Alvarez-Buylla, Arturo
Format: Article
Language:English
Subjects:
Animals
Brain Tissue Transplantation
Calbindin 2
Calbindins
Cell Differentiation - physiology
Cell Lineage - physiology
Cell Movement - physiology
Cells, Cultured
Dopamine - metabolism
gamma-Aminobutyric Acid - metabolism
Graft Survival - physiology
Homeodomain Proteins - metabolism
Interneurons - cytology
Interneurons - metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Olfactory Bulb - cytology
Olfactory Bulb - embryology
Olfactory Bulb - metabolism
S100 Calcium Binding Protein G - metabolism
Stem Cells - cytology
Stem Cells - metabolism
Telencephalon - cytology
Telencephalon - embryology
Telencephalon - metabolism
Transcription Factors - metabolism
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Summary:The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin+, calbindin+, and dopaminergic (TH+) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain, but they matured and were eliminated at different rates. The embryonic lateral ganglionic eminence (LGE) is thought to be the site of origin of postnatal SVZ neural progenitors. Consistently, grafts of the embryonic LGE into the adult brain SVZ generated many OB interneurons, including TH+ and calbindin+ periglomerular interneurons. However, calretinin+ cells were not produced from these LGE grafts. Surprisingly, pallial and septal embryonic progenitors transplanted into the adult brain SVZ also resulted in the generation of OB interneurons, including calretinin+ cells. A subset of Dlx2+ OB interneurons was derived from cells expressing Emx1, a transcription factor largely restricted to the pallium during development. Emx1 lineage-derived cells contributed a substantial portion of GABAergic cells in the OB, including calretinin+ interneurons. This is in contrast to cortex, in which Emx1 lineage-derived cells do not differentiate into GABAergic neurons. Our results suggest that some OB interneurons are derived from progenitors outside the LGE and that precursors expressing what has classically been considered a pallial transcription factor generate GABAergic interneurons.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.0254-07.2007