SNP haplotyping technique for evaluation of MGP 5′ UTR power in osteoblast cells

Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), a...

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Bibliographic Details
Published in:SpringerPlus 2016-06, Vol.5 (1), p.711-711, Article 711
Main Authors: Roustazadeh, Abazar, Monavari, Seyed Hamidreza, Hosseini Fard, Seyed Reza, Hassanpour, Parisa, Yarnazari, Amaneh, Najafi, Mohammad
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
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Summary:Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells.
ISSN:2193-1801
2193-1801
DOI:10.1186/s40064-016-2329-8