Genomic Analysis Using a Yeast Artificial Chromosome Library with Mouse DNA Inserts

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individuall...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2456-2460
Main Authors: Rossi, Janice M., Burke, David T., Jimmy C. M. Leung, Koos, David S., Chen, Hsiuchen, Tilghman, Shirley M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cellular biology
Chromosome Mapping
Chromosomes
Chromosomes, Fungal
Deoxyribonucleic acid
Diverse techniques
DNA
DNA - genetics
DNA - isolation & purification
DNA probes
Exons
Female
Fundamental and applied biological sciences. Psychology
Gels
Gene Library
Genetic loci
Genetic Markers
Genetic screening
Genetics
Genome
Genomics
Libraries
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Molecular Sequence Data
Multidisciplinary Sciences
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Rodents
Saccharomyces cerevisiae - genetics
Science & Technology
Science & Technology - Other Topics
Spleen - physiology
Yeasts
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Description
Summary:A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.6.2456