Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress

Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate, and severe) during the elongation stage and gene expression was...

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Bibliographic Details
Published in:Scientific reports 2016-05, Vol.6 (1), p.25698-25698, Article 25698
Main Authors: Li, Changning, Nong, Qian, Solanki, Manoj Kumar, Liang, Qiang, Xie, Jinlan, Liu, Xiaoyan, Li, Yijie, Wang, Weizan, Yang, Litao, Li, Yangrui
Format: Article
Language:English
Subjects:
Droughts
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Ontology
Plant Leaves - genetics
Plant Leaves - growth & development
Plant Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
Saccharum - genetics
Saccharum - growth & development
Signal Transduction - genetics
Stress, Physiological - genetics
Water - metabolism
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Summary:Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate, and severe) during the elongation stage and gene expression was analyzed using microarray technology. Physiological parameters of sugarcane showed significant alterations in response to drought stress. Based on the expression profile of 15,593 sugarcane genes, 1,501 (9.6%) genes were differentially expressed under different water-level treatments; 821 genes were upregulated and 680 genes were downregulated. A gene similarity analysis showed that approximately 62.6% of the differentially expressed genes shared homology with functional proteins. In a Gene Ontology (GO) analysis, 901 differentially expressed genes were assigned to 36 GO categories. Moreover, 325 differentially expressed genes were classified into 101 pathway categories involved in various processes, such as the biosynthesis of secondary metabolites, ribosomes, carbon metabolism, etc. In addition, some unannotated genes were detected; these may provide a basis for studies of water-deficit tolerance. The reliability of the observed expression patterns was confirmed by RT-PCR. The results of this study may help identify useful genes for improving drought tolerance in sugarcane.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep25698