HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme

Momilactones, which are diterpenoid phytoalexins with antimicrobial and allelopathic functions, have been found only in rice and the moss Hypnum plumaeforme. Although these two evolutionarily distinct plant species are thought to produce momilactones as a chemical defence, the momilactone biosynthet...

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Published in:Scientific reports 2016-05, Vol.6 (1), p.25316-25316, Article 25316
Main Authors: Okada, Kazunori, Kawaide, Hiroshi, Miyamoto, Koji, Miyazaki, Sho, Kainuma, Ryosuke, Kimura, Honoka, Fujiwara, Kaoru, Natsume, Masahiro, Nojiri, Hideaki, Nakajima, Masatoshi, Yamane, Hisakazu, Hatano, Yuki, Nozaki, Hiroshi, Hayashi, Ken-Ichiro
Format: Article
Language:English
Subjects:
Alkyl and Aryl Transferases - genetics
Alkyl and Aryl Transferases - metabolism
Biosynthesis
Bryophyta - enzymology
Bryophyta - genetics
Bryophyta - metabolism
Chemical defense
Diterpenes - metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Jasmonic acid
Mosses
Phytoalexins
Plants
Sequence Analysis, DNA
Signal transduction
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Summary:Momilactones, which are diterpenoid phytoalexins with antimicrobial and allelopathic functions, have been found only in rice and the moss Hypnum plumaeforme. Although these two evolutionarily distinct plant species are thought to produce momilactones as a chemical defence, the momilactone biosynthetic pathway in H. plumaeforme has been unclear. Here, we identified a gene encoding syn-pimara-7,15-diene synthase (HpDTC1) responsible for the first step of momilactone biosynthesis in the moss. HpDTC1 is a bifunctional diterpene cyclase that catalyses a two-step cyclization reaction of geranylgeranyl diphosphate to syn-pimara-7,15-diene. HpDTC1 transcription was up-regulated in response to abiotic and biotic stress treatments. HpDTC1 promoter-GUS analysis in transgenic Physcomitrella patens showed similar transcriptional responses as H. plumaeforme to the stresses, suggesting that a common response system to stress exists in mosses. Jasmonic acid (JA), a potent signalling molecule for inducing plant defences, could not activate HpDTC1 expression. In contrast, 12-oxo-phytodienoic acid, an oxylipin precursor of JA in vascular plants, enhanced HpDTC1 expression and momilactone accumulation, implying that as-yet-unknown oxylipins could regulate momilactone biosynthesis in H. plumaeforme. These results demonstrate the existence of an evolutionarily conserved chemical defence system utilizing momilactones and suggest the molecular basis of the regulation for inductive production of momilactones in H. plumaeforme.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep25316