Application of hollow fiber vitrification for cryopreservation of bovine early cleavage stage embryos and porcine morula-blastomeres

A novel hollow fiber vitrification (HFV) method was applied to materials that have previously been difficult to cryopreserve, thereby expanding the potential application of this method. The results showed that zona-free porcine morulae and their isolated blastomeres remained viable even after vitrif...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2016, Vol.62(2), pp.219-223
Main Authors: UCHIKURA, Ayuko, MATSUNARI, Hitomi, NAKANO, Kazuaki, HATAE, Shota, NAGASHIMA, Hiroshi
Format: Article
Language:English
Subjects:
Bovine embryos
Cryopreservation
Hollow fiber vitrification (HFV)
Porcine morula-blastomeres
Technology Report
Zona-free embryos
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Summary:A novel hollow fiber vitrification (HFV) method was applied to materials that have previously been difficult to cryopreserve, thereby expanding the potential application of this method. The results showed that zona-free porcine morulae and their isolated blastomeres remained viable even after vitrification. The rate of development to blastocysts after vitrification was similar for zona-free and zona-intact morulae (21/23, 91.3% for both). Vitrified blastomeres had a developmental potential equal to that of non-vitrified blastomeres (blastocyst formation rate after reaggregation: 16/17, 94.1% for both). The HFV method was also effective for the cryopreservation of in vitro matured/fertilized bovine embryos at the 2- to 4-cell, 8- to 16-cell and morula stages. The blastocyst formation rates of vitrified embryos (66.1–82.5%) were similar to those of non-vitrified embryos (74.5–82.5%). These results indicate that this novel HFV method is an effective tool for embryo cryopreservation that can enhance current practices in reproductive biology.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.2015-162