LRRC31 is induced by IL-13 and regulates kallikrein expression and barrier function in the esophageal epithelium

Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of the esophagus featuring increased esophageal interleukin-13 (IL-13) levels and impaired barrier function. Herein, we investigated leucine-rich repeat-containing protein 31 (LRRC31) in human EoE esophageal tissue and IL-13-treated...

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Bibliographic Details
Published in:Mucosal immunology 2016-05, Vol.9 (3), p.744-756
Main Authors: D'Mello, R J, Caldwell, J M, Azouz, N P, Wen, T, Sherrill, J D, Hogan, S P, Rothenberg, M E
Format: Article
Language:English
Subjects:
Adult
Biological Transport
Cell Differentiation
Cell Line
Chemokines
Dextrans - pharmacokinetics
Electric Impedance
Electrical resistivity
Eosinophilia
Eosinophilic Esophagitis - immunology
Epithelial cells
Epithelium
Epithelium - metabolism
Epithelium - pathology
Esophageal diseases
Esophagitis
Esophagus
Esophagus - immunology
Esophagus - pathology
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluorescein-5-isothiocyanate - pharmacokinetics
Gene expression
Gene Expression Regulation
Humans
Inflammatory diseases
Interleukin 1
Interleukin 13
Interleukin-13 - genetics
Interleukin-13 - metabolism
Kallikrein
Kallikreins - genetics
Kallikreins - metabolism
Leucine
Leucine-Rich Repeat Proteins
Leukocytes (eosinophilic)
Mucosa
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Proteins
Proteins - genetics
Proteins - metabolism
RNA, Small Interfering - genetics
Serine
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Summary:Eosinophilic esophagitis (EoE) is an allergic inflammatory disease of the esophagus featuring increased esophageal interleukin-13 (IL-13) levels and impaired barrier function. Herein, we investigated leucine-rich repeat-containing protein 31 (LRRC31) in human EoE esophageal tissue and IL-13-treated esophageal epithelial cells. LRRC31 had basal mRNA expression in colonic and airway mucosal epithelium. Esophageal LRRC31 mRNA and protein increased in active EoE and strongly correlated with esophageal eosinophilia and IL13 and CCL26 (chemokine (C-C motif) ligand 26) mRNA expression. IL-13 treatment increased LRRC31 mRNA and protein in air-liquid interface-differentiated esophageal epithelial cells (EPC2s). At baseline, differentiated LRRC31-overexpressing EPC2s had increased barrier function (1.9-fold increase in transepithelial electrical resistance (P
ISSN:1933-0219
1935-3456
DOI:10.1038/mi.2015.98