Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing

Highlights • Deep sequencing has potential as an improved adventitious virus screening method. • 15 laboratories sequenced a common reagent containing 25 target viruses. • 6 viruses were detected by all lab, the remainder were detected by 4–14 labs. • A wide range of sample preparation and bioinform...

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Bibliographic Details

Published in:	Vaccine 2016-04, Vol.34 (17), p.2035-2043
Main Authors:	Mee, Edward T, Preston, Mark D, Minor, Philip D, Schepelmann, Silke
Format:	Article
Language:	eng
Subjects:	Acids Adventitious virus Allergy and Immunology Biological Products - standards Cell culture Collaboration Collaborative study Committees Cytomegalovirus Deep sequencing Drug Contamination - prevention & control Genomes High-Throughput Nucleotide Sequencing Informatics Laboratories Manufacturers Methods Pathogens Proprietary Reference material Reference materials Reference Standards Vaccine Vaccines - standards Viruses Viruses - isolation & purification
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