Single amino acid change in the helicase domain of the putative RNA replicase of turnip crinkle virus alters symptom intensification by virulent satellites

The virulent satellite [satellite C (sat C)] of turnip crinkle virus (TCV) is a small pathogenic RNA that intensifies symptoms in TCV-infected turnip plants (Brassica campestris). The virulence of sat C is determined by properties of the satellite itself and is influenced by the helper virus. Sympto...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992, Vol.89 (1), p.309-313
Main Authors: Collmer, C.W, Stenzler, L, Chen, X.M, Fay, N, Hacker, D, Howell, S.H
Format: Article
Language:English
Subjects:
Amino acids
Artificial satellites
Base Sequence
Biological and medical sciences
Botany
Brassica rapa subsp. campestris
Chemical bases
Cloning, Molecular
Complementary DNA
DNA Mutational Analysis
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genomes
Helper viruses
Helper Viruses - genetics
Immunobiology
Molecular Sequence Data
Multidisciplinary Sciences
Myeloid cells: ontogeny, maturation, markers, receptors
nucleotides
Oligodeoxyribonucleotides - chemistry
Plant Diseases - genetics
plant viruses
Plant Viruses - genetics
Plasmids
Polymerase Chain Reaction
Protoplasts
RNA
RNA - genetics
RNA Replicase - chemistry
RNA, Satellite
RNA, Viral - genetics
Satellite RNA
Science & Technology
Science & Technology - Other Topics
Turnip crinkle virus
Vegetables
virulence
Viruses
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Description
Summary:The virulent satellite [satellite C (sat C)] of turnip crinkle virus (TCV) is a small pathogenic RNA that intensifies symptoms in TCV-infected turnip plants (Brassica campestris). The virulence of sat C is determined by properties of the satellite itself and is influenced by the helper virus. Symptoms produced in infections with sat C differ in severity depending on the helper virus. The TCV-JI helper virus produces more severe symptoms than the TCV-B helper virus when inoculated with sat C. To rind determinants in the TCV helper virus genome that affect satellite virulence, the TCV-JI genome was cloned and the sequence was compared to the TCV-B genome. The genomes were found to differ by only five base changes, and only one of the base changes, at nucleotide position 1025, produced an amino acid change, an aspartic acid leads to glycine in the putative viral replicase. A chimeric TCV genome (TCV-B/JI) containing four of the rive base changes (including the base change at position 1025) and a mutant TCV-B genome (TCV-B1205G) containing a single base substitution at position 1025 converted the TCV-B genome into a form that produces severe symptoms with sat C. The base change at position 1025 is located in the helicase region of the putative viral replicase, and symptom intensification appears to result from differences in the rate of replication of the satellite supported by the two helper viruses.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.1.309