Fish oil supplementation suppresses resistance exercise and feeding‐induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men

Fish oil (FO) supplementation potentiates muscle protein synthesis (MPS) in response to a hyperaminoacidemic–hyperinsulinemic infusion. Whether FO supplementation potentiates MPS in response to protein ingestion or when protein ingestion is combined with resistance exercise (RE) remains unknown. In...

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Bibliographic Details
Published in:	Physiological reports 2016-03, Vol.4 (6), p.e12715-n/a
Main Authors:	McGlory, Chris, Wardle, Sophie L., Macnaughton, Lindsay S., Witard, Oliver C., Scott, Fraser, Dick, James, Bell, J. Gordon, Phillips, Stuart M., Galloway, Stuart D. R., Hamilton, D. Lee, Tipton, Kevin D.
Format:	Article
Language:	eng
Subjects:	Amino acids AMP-Activated Protein Kinases - metabolism Anabolic Agents - administration & dosage Biopsy Coconut oil Dietary Proteins - administration & dosage Dietary Supplements Endurance and Performance Fish oil Fish oils Fish Oils - administration & dosage Fitness training programs Humans Lipid composition Male Medical screening Metabolism Muscle Metabolism Muscle Proteins - biosynthesis Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Musculoskeletal system myofibrillar muscle protein synthesis Myofibrils - drug effects Myofibrils - metabolism Nutrition Older people Original Research p70S6K1 Phenylalanine Phospholipids - metabolism Phosphorylation Physiology Protein biosynthesis Protein Biosynthesis - drug effects Protein composition Protein synthesis Proteins Proto-Oncogene Proteins c-akt - metabolism resistance exercise Resistance Training Ribosomal Protein S6 Kinases, 70-kDa - metabolism Scotland Signal Transduction - drug effects Signalling Pathways Skeletal Muscle Studies Supplements Time Factors Treatment Outcome Whey protein Young Adult Young adults
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Summary:	Fish oil (FO) supplementation potentiates muscle protein synthesis (MPS) in response to a hyperaminoacidemic–hyperinsulinemic infusion. Whether FO supplementation potentiates MPS in response to protein ingestion or when protein ingestion is combined with resistance exercise (RE) remains unknown. In a randomized, parallel group design, 20 healthy males were randomized to receive 5 g/day of either FO or coconut oil control (CO) for 8 weeks. After supplementation, participants performed a bout of unilateral RE followed by ingestion of 30 g of whey protein. Skeletal muscle biopsies were obtained before and after supplementation for assessment of muscle lipid composition and relevant protein kinase activities. Infusion of l‐[ring‐13C6] phenylalanine was used to measure basal myofibrillar MPS at rest (REST), in a nonexercised leg following protein ingestion (FED) and following RE and protein ingestion (FEDEX). MPS was significantly elevated above REST during FEDEX in both the FO and CO groups, but there was no effect of supplementation. There was a significant increase in MPS in both groups above REST during FED but no effect of supplementation. Supplementation significantly decreased panPKB activity at REST in the FO group but not the CO group. There was a significant increase from REST at post‐RE for PKB and AMPKα2 activity in the CO group but not in the FO group. In FEDEX, there was a significant increase in p70S6K1 activity from REST at 3 h in the CO group only. These data highlight that 8 weeks of FO supplementation alters kinase signaling activity in response to RE plus protein ingestion without influencing MPS. Fish oil supplementation has been shown to potentiate rates of muscle protein synthesis (MPS) in response to a hyperaminocidemic–hyperinsulinemic infusion in both young and older adults. Here, we show that 8 weeks of fish oil supplementation does not significantly alter MPS following the oral ingestion of whey protein or when whey protein ingestion is preceded by a bout of unilateral resistance exercise in young healthy men.
ISSN:	2051-817X 2051-817X