Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall

During inflammation, circulating neutrophils roll on, and eventually tether to, the endothelial lining of blood vessels, allowing them to exit the bloodstream and enter the surrounding tissue to target pathogens. This process is mediated by the selectin family of adhesion proteins expressed by endot...

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Bibliographic Details
Published in:Cellular and molecular bioengineering 2016-03, Vol.9 (1), p.85-95
Main Authors: Rocheleau, Anne D., Wang, Weiwei, King, Michael R.
Format: Article
Language:English
Subjects:
Adhesion
Bioengineering
Biological and Medical Physics
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Biophysics
Blood vessels
Cascades
Cell adhesion & migration
Cell Biology
Cellular
Circulating
Contact
Endothelium
Engineering
Fluid mechanics
Inflammation
Proteins
Transport
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Summary:During inflammation, circulating neutrophils roll on, and eventually tether to, the endothelial lining of blood vessels, allowing them to exit the bloodstream and enter the surrounding tissue to target pathogens. This process is mediated by the selectin family of adhesion proteins expressed by endothelial cells. Interestingly, only 10% of activated, migrating neutrophils transmigrate into the extravascular space; the other 90% detach from the wall and rejoin the blood flow. Neutrophils extrude pseudopods during the adhesion cascade; however, the transport behavior of this unique cell geometry has not been previously addressed. In this study, a three-dimensional computational model was applied to neutrophils with pseudopodial extensions to study the effect of cell shape on the hydrodynamic transport of neutrophils. The collision time, contact area, contact force, and collision frequency were analyzed as a function of pseudopod length. It was found that neutrophils experience more frequent collisions compared to prolate spheroids of equal volume and length. Longer pseudopods and lower shear rates increase the collision time integral contact area, a predictor of binding potential. Our results indicate that contact between the neutrophil and the vessel wall is found to be focused predominantly on the pseudopod tip.
ISSN:1865-5025
1865-5033
DOI:10.1007/s12195-015-0420-9