Principles of connectivity among morphologically defined cell types in adult neocortex

Since the work of Ramón y Cajal in the late 19th and early 20th centuries, neuroscientists have speculated that a complete understanding of neuronal cell types and their connections is key to explaining complex brain functions. However, a complete census of the constituent cell types and their wirin...

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Published in:Science (American Association for the Advancement of Science) 2015-11, Vol.350 (6264), p.1055-1055
Main Authors: Jiang, Xiaolong, Shen, Shan, Cadwell, Cathryn R., Berens, Philipp, Sinz, Fabian, Ecker, Alexander S., Patel, Saumil, Tolias, Andreas S.
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Avidin
Biotin
Brain
Census
Cortexes
GABAergic Neurons - classification
GABAergic Neurons - cytology
GABAergic Neurons - physiology
Interneurons - classification
Interneurons - cytology
Interneurons - physiology
Joints
Mice
Neocortex - cytology
Neocortex - physiology
Neural Inhibition
Neural Pathways - cytology
Neural Pathways - physiology
Neurons
Patch-Clamp Techniques
Peroxidase
Pyramidal Cells - cytology
Pyramidal Cells - physiology
Recording
RESEARCH ARTICLE SUMMARY
Staining and Labeling
Synapses
Synapses - physiology
Synapses - ultrastructure
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Summary:Since the work of Ramón y Cajal in the late 19th and early 20th centuries, neuroscientists have speculated that a complete understanding of neuronal cell types and their connections is key to explaining complex brain functions. However, a complete census of the constituent cell types and their wiring diagram in mature neocortex remains elusive. By combining octuple whole-cell recordings with an optimized avidin-biotin-peroxidase staining technique, we carried out a morphological and electrophysiological census of neuronal types in layers 1, 2/3, and 5 of mature neocortex and mapped the connectivity between more than 11,000 pairs of identified neurons. We categorized 15 types of interneurons, and each exhibited a characteristic pattern of connectivity with other interneuron types and pyramidal cells. The essential connectivity structure of the neocortical microcircuit could be captured by only a few connectivity motifs.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aac9462