Direct Reprogramming of Human Amniotic Fluid Stem Cells by OCT4 and Application in Repairing of Cerebral Ischemia Damage

Amniotic fluid stem cells (AFSCs) are a type of fetal stem cell whose stemness encompasses both embryonic and adult stem cells, suggesting that they may be easily and efficiently reprogrammed into induced pluripotent stem cells (iPSCs). To further simplify the reprogramming process, the creation of...

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Bibliographic Details
Published in:International journal of biological sciences 2016-01, Vol.12 (5), p.558-568
Main Authors: Qin, Mingde, Chen, Ruihua, Li, Hong, Liang, Hansi, Xue, Qun, Li, Fang, Chen, Ying, Zhang, Xueguang
Format: Article
Language:English
Subjects:
Amniotic Fluid - cytology
Animals
Brain Ischemia - metabolism
Brain Ischemia - therapy
Cell Differentiation - genetics
Cell Differentiation - physiology
Cells, Cultured
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Male
Mice
Mice, Inbred BALB C
Octamer Transcription Factor-3 - genetics
Octamer Transcription Factor-3 - metabolism
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Rats
Rats, Wistar
Research Paper
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Summary:Amniotic fluid stem cells (AFSCs) are a type of fetal stem cell whose stemness encompasses both embryonic and adult stem cells, suggesting that they may be easily and efficiently reprogrammed into induced pluripotent stem cells (iPSCs). To further simplify the reprogramming process, the creation of AFSC-derived iPSCs using a single factor is desirable. Here we report the generation of one-factor human AFSC-iPSCs (AiPSCs) from human AFSCs by ectopic expression of the transcription factor OCT4. Just like human embryonic stem cells, AiPSCs exhibited similar epigenetic status, global gene expression profiles, teratoma formation and in vitro & in vivo pluripotency. Our results indicate that the OCT4 is necessary and sufficient to directly reprogram human AFSCs into pluripotent AiPSCs. Moreover, reflecting the similar memory characteristics of AFSCs and neural stem cells, we show that AiPSC membrane-derived vesicles (MVs) repair cerebral ischemia damage. We anticipate that the successful generation of one-factor AiPSCs will facilitate the creation of patient-specific pluripotent stem cells without the need for transgenic expression of oncogenes. Moreover, MVs from tissue-specific AiPSCs have potential in tissue repair, representing a novel application of iPSCs.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.11051