Increases in skeletal muscle ATGL and its inhibitor G0S2 following 8 weeks of endurance training in metabolically different rat skeletal muscles

Adipose triglyceride lipase (ATGL) catalyzes the rate-limiting removal of the first fatty acid from a triglyceride. ATGL is activated by comparative gene identification-58 and inhibited by G(0)/G(1) switch gene-2 protein (G0S2). Research in other tissues and cell culture indicates that inhibition is...

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Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2016-01, Vol.310 (2), p.R125-R133
Main Authors: Turnbull, Patrick C, Longo, Amanda B, Ramos, Sofhia V, Roy, Brian D, Ward, Wendy E, Peters, Sandra J
Format: Article
Language:English
Subjects:
Animals
Cell Cycle Proteins - metabolism
Electric Stimulation
Fatty acids
Lipase - metabolism
Lipolysis
Male
Metabolism
Mitochondria, Muscle - metabolism
Muscle Contraction
Muscle, Skeletal - enzymology
Muscle, Skeletal - innervation
Musculoskeletal system
Obesity, Diabetes and Energy Homeostasis
Oxidation-Reduction
Physical Activity and Inactivity
Physical Conditioning, Animal
Physical Endurance
Proteins
Rats, Sprague-Dawley
Rodents
Sciatic Nerve - physiology
Time Factors
Triglycerides
Triglycerides - metabolism
Up-Regulation
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Summary:Adipose triglyceride lipase (ATGL) catalyzes the rate-limiting removal of the first fatty acid from a triglyceride. ATGL is activated by comparative gene identification-58 and inhibited by G(0)/G(1) switch gene-2 protein (G0S2). Research in other tissues and cell culture indicates that inhibition is dependent on relative G0S2-to-ATGL protein content. G0S2 may also have several roles within mitochondria; however, this has yet to be observed in skeletal muscle. The purpose of this study was to determine if muscle G0S2 relative to ATGL content would decrease to facilitate intramuscular lipolysis following endurance training. Male Sprague-Dawley rats (n = 10; age 51-53 days old) were progressively treadmill trained at a 10% incline for 8 wk ending with 25 m/min for 1 h compared with control. Sciatic nerve stimulation for hind-limb muscle contraction (and lipolysis) was administered for 30 min to one leg, leaving the opposing leg as a resting control. Soleus (SOL), red gastrocnemius (RG), and white gastrocnemius were excised from both legs following stimulation or control. ATGL protein increased in all trained muscles. Unexpectedly, G0S2 protein was greater in the trained SOL and RG. In RG-isolated mitochondria, G0S2 also increased with training, yet mitochondrial G0S2 content was unaltered with acute contraction; therefore, any role of G0S2 in the mitochondria does not appear to be acutely mediated by content alone. In summary, G0S2 increased with training in oxidative muscles and mitochondria but not following acute contraction, suggesting that inhibition is not through relative G0S2-to-ATGL content but through more complicated intracellular mechanisms.
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.00062.2015