Assessment of red blood cell deformability in type 2 diabetes mellitus and diabetic retinopathy by dual optical tweezers stretching technique

Assessment of red blood cell deformability in type 2 diabetes mellitus and diabetic retinopathy by dual optical tweezers stretching technique

A pilot cross sectional study was conducted to investigate the role of red blood cells (RBC) deformability in type 2 diabetes mellitus (T2DM) without and with diabetic retinopathy (DR) using a dual optical tweezers stretching technique. A dual optical tweezers was made by splitting and recombining a...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-03, Vol.6 (1), p.15873-15873, Article 15873
Main Authors: Agrawal, Rupesh, Smart, Thomas, Nobre-Cardoso, João, Richards, Christopher, Bhatnagar, Rhythm, Tufail, Adnan, Shima, David, H Jones, Phil, Pavesio, Carlos
Format: Article
Language:eng
Subjects:
Blood
Blood cells
Cell Shape - physiology
Cross-Sectional Studies
Deformability
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2 - physiopathology
Diabetic retinopathy
Diabetic Retinopathy - physiopathology
Erythrocyte Deformability - physiology
Erythrocytes
Erythrocytes - cytology
Female
Hematology - instrumentation
Hematology - methods
Humans
Male
Microscopy, Video
Middle Aged
Optical Tweezers
Pilot Projects
Regression Analysis
Retinopathy
Splitting
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A pilot cross sectional study was conducted to investigate the role of red blood cells (RBC) deformability in type 2 diabetes mellitus (T2DM) without and with diabetic retinopathy (DR) using a dual optical tweezers stretching technique. A dual optical tweezers was made by splitting and recombining a single Nd:YAG laser beam. RBCs were trapped directly (i.e., without microbead handles) in the dual optical tweezers where they were observed to adopt a "side-on" orientation. RBC initial and final lengths after stretching were measured by digital video microscopy, and a Deformability index (DI) calculated. Blood from 8 healthy controls, 5 T2DM and 7 DR patients with respective mean age of 52.4 yrs, 51.6 yrs and 52 yrs was analysed. Initial average length of RBCs for control group was 8.45 ± 0.25 μm, 8.68 ± 0.49 μm for DM RBCs and 8.82 ± 0.32 μm for DR RBCs (p < 0.001). The DI for control group was 0.0698 ± 0.0224, and that for DM RBCs was 0.0645 ± 0.03 and 0.0635 ± 0.028 (p < 0.001) for DR group. DI was inversely related to basal length of RBCs (p =  .02). DI of RBC from DM and DR patients was significantly lower in comparison with normal healthy controls. A dual optical tweezers method can hence be reliably used to assess RBC deformability.
ISSN:2045-2322
2045-2322