Allocation of the S-genome chromosomes of Aegilops variabilis Eig. carrying powdery mildew resistance in triticale (× Triticosecale Wittmack)

It has been hypothesized that the powdery mildew adult plant resistance (APR) controlled by the Pm13 gene in Aegilops longissima Schweinf. & Muschl. (SË¡SË¡) has been evolutionary transferred to Aegilops variabilis Eig. (UUSS). The molecular marker analysis and the visual evaluation of powdery m...

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Bibliographic Details
Published in:Protoplasma 2016-03, Vol.253 (2), p.329-343
Main Authors: Kwiatek, M, J. Belter, M. Majka, H. Wiśniewska
Format: Article
Language:English
Subjects:
Aegilops
Aegilops longissima
Aegilops peregrina var. peregrina
Biomedical and Life Sciences
Blumeria graminis
Cell Biology
chromosome pairing
chromosomes
Chromosomes, Plant - genetics
Disease Resistance - genetics
fluorescence in situ hybridization
genes
Genetic Predisposition to Disease
Genome, Plant
hybrids
Life Sciences
marker-assisted selection
mature plants
Meiosis
metaphase
Mitosis
Original
Original Article
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Sciences
pollen
powdery mildew
Secale cereale
statistical analysis
substitution lines
triticale
Triticale - cytology
Triticale - genetics
Triticale - microbiology
Triticosecale
Zoology
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Summary:It has been hypothesized that the powdery mildew adult plant resistance (APR) controlled by the Pm13 gene in Aegilops longissima Schweinf. & Muschl. (SˡSˡ) has been evolutionary transferred to Aegilops variabilis Eig. (UUSS). The molecular marker analysis and the visual evaluation of powdery mildew symptoms in Ae. variabilis and the Ae. variabilis × Secale cereale amphiploid forms (2n = 6x = 42, UUSSRR) showed the presence of product that corresponded to Pm13 marker and the lower infection level compared to susceptible model, respectively. This study also describes the transfer of Ae. variabilis Eig. (2n = 4x = 28, UᵛUᵛSᵛSᵛ) chromosomes, carrying powdery mildew resistance, into triticale (× Triticosecale Wittm., 2n = 6x = 42, AABBRR) using Ae. variabilis × S. cereale amphiploid forms. The individual chromosomes of Ae. variabilis, triticale ‘Lamberto’ and hybrids were characterized by genomic and fluorescence in situ hybridization (GISH/FISH). The chromosome configurations of obtained hybrid forms were studied at first metaphase of meiosis of pollen mother cells (PMCs) using GISH. The statistical analysis showed that the way of S-genome chromosome pairing and transmission to subsequent hybrid generations was diploid-like and had no influence on chromosome pairing of triticale chromosomes. The cytogenetic study of hybrid forms were supported by the marker-assisted selection using Pm13 marker and visual evaluation of natural infection by Blumeria graminis, that allowed to select the addition or substitution lines of hybrids carrying chromosome 3Sᵛ which were tolerant to the powdery mildew infection.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-015-0813-6