Juvenile Hormone Activates the Transcription of Cell-division-cycle 6 (Cdc6) for Polyploidy-dependent Insect Vitellogenesis and Oogenesis

Although juvenile hormone (JH) is known to prevent insect larval metamorphosis and stimulate adult reproduction, the molecular mechanisms of JH action in insect reproduction remain largely unknown. Earlier, we reported that the JH-receptor complex, composed of methoprene-tolerant and steroid recepto...

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Bibliographic Details
Published in:The Journal of biological chemistry 2016-03, Vol.291 (10), p.5418-5427
Main Authors: Wu, Zhongxia, Guo, Wei, Xie, Yingtian, Zhou, Shutang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Line
DNA replication
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila melanogaster - physiology
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Fat Body - metabolism
Female
Gene Regulation
Grasshoppers - genetics
Grasshoppers - metabolism
Grasshoppers - physiology
insect
juvenile hormone (JH)
Juvenile Hormones - metabolism
Molecular Sequence Data
Ovarian Follicle - metabolism
Polyploidy
reproduction
Transcriptional Activation
Vitellins - genetics
Vitellins - metabolism
Vitellogenesis
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Summary:Although juvenile hormone (JH) is known to prevent insect larval metamorphosis and stimulate adult reproduction, the molecular mechanisms of JH action in insect reproduction remain largely unknown. Earlier, we reported that the JH-receptor complex, composed of methoprene-tolerant and steroid receptor co-activator, acts on mini-chromosome maintenance (Mcm) genes Mcm4 and Mcm7 to promote DNA replication and polyploidy for the massive vitellogenin (Vg) synthesis required for egg production in the migratory locust (Guo, W., Wu, Z., Song, J., Jiang, F., Wang, Z., Deng, S., Walker, V. K., and Zhou, S. (2014) PLoS Genet. 10, e1004702). In this study we have investigated the involvement of cell-division-cycle 6 (Cdc6) in JH-dependent vitellogenesis and oogenesis, as Cdc6 is essential for the formation of prereplication complex. We demonstrate here that Cdc6 is expressed in response to JH and methoprene-tolerant, and Cdc6 transcription is directly regulated by the JH-receptor complex. Knockdown of Cdc6 inhibits polyploidization of fat body and follicle cells, resulting in the substantial reduction of Vg expression in the fat body as well as severely impaired oocyte maturation and ovarian growth. Our data indicate the involvement of Cdc6 in JH pathway and a pivotal role of Cdc6 in JH-mediated polyploidization, vitellogenesis, and oogenesis.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M115.698936