Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography

X-ray fluorescence computed tomography (XFCT) is a technique that can identify, quantify, and locate elements within objects by detecting x-ray fluorescence (characteristic x-rays) stimulated by an excitation source, typically derived from a synchrotron. However, the use of a synchrotron limits prac...

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Bibliographic Details
Published in:Scientific reports 2016-02, Vol.6 (1), p.22079-22079, Article 22079
Main Authors: Manohar, Nivedh, Reynoso, Francisco J, Diagaradjane, Parmeswaran, Krishnan, Sunil, Cho, Sang Hyun
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Computed tomography
Contrast Media - chemistry
Contrast Media - pharmacology
Fluorescence
Gold
Gold - chemistry
Gold - pharmacology
Humans
Male
Mass spectroscopy
Medical imaging
Metal Nanoparticles - chemistry
Mice
Nanoparticles
Neoplasms, Experimental - diagnostic imaging
Probes
Prostatic Neoplasms - diagnostic imaging
Spatial distribution
Spectrometry, X-Ray Emission - methods
Tomography, X-Ray Computed - methods
X-rays
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Summary:X-ray fluorescence computed tomography (XFCT) is a technique that can identify, quantify, and locate elements within objects by detecting x-ray fluorescence (characteristic x-rays) stimulated by an excitation source, typically derived from a synchrotron. However, the use of a synchrotron limits practicality and accessibility of XFCT for routine biomedical imaging applications. Therefore, we have developed the ability to perform XFCT on a benchtop setting with ordinary polychromatic x-ray sources. Here, we report our postmortem study that demonstrates the use of benchtop XFCT to accurately image the distribution of gold nanoparticles (GNPs) injected into a tumor-bearing mouse. The distribution of GNPs as determined by benchtop XFCT was validated using inductively coupled plasma mass spectrometry. This investigation shows drastically enhanced sensitivity and specificity of GNP detection and quantification with benchtop XFCT, up to two orders of magnitude better than conventional x-ray CT. The results also reaffirm the unique capabilities of benchtop XFCT for simultaneous determination of the spatial distribution and concentration of nonradioactive metallic probes, such as GNPs, within the context of small animal imaging. Overall, this investigation identifies a clear path toward in vivo molecular imaging using benchtop XFCT techniques in conjunction with GNPs and other metallic probes.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep22079