The Pangenome of the Anticarsia gemmatalis Multiple Nucleopolyhedrovirus (AgMNPV)

The alphabaculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is the world's most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations of Anticarsia gemmatalis (Velvetbean caterpillar) in soybean...

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Bibliographic Details
Published in:Genome biology and evolution 2015-11, Vol.8 (1), p.94-108
Main Authors: Brito, Anderson Fernandes de, Braconi, Carla Torres, Weidmann, Manfred, Dilcher, Meik, Alves, João Marcelo Pereira, Gruber, Arthur, Zanotto, Paolo Marinho de Andrade
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - genetics
Base Sequence
Genome, Viral
Genomic Instability
Lepidoptera - virology
Molecular Sequence Data
Open Reading Frames
Polymorphism, Genetic
Recombination, Genetic
Ubiquitins - genetics
Viral Proteins - genetics
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Summary:The alphabaculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is the world's most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations of Anticarsia gemmatalis (Velvetbean caterpillar) in soybean crops. During this period, genetic studies identified several variable loci in the AgMNPV; however, most of them were not characterized at the sequence level. In this study we report a full genome comparison among 17 wild-type isolates of AgMNPV. We found the pangenome of this virus to contain at least 167 hypothetical genes, 151 of which are shared by all genomes. The gene bro-a that might be involved in host specificity and carrying transporter is absent in some genomes, and new hypothetical genes were observed. Among these genes there is a unique rnf12-like gene, probably implicated in ubiquitination. Events of gene fission and fusion are common, as four genes have been observed as single or split open reading frames. Gains and losses of genomic fragments (from 20 to 900 bp) are observed within tandem repeats, such as in eight direct repeats and four homologous regions. Most AgMNPV genes present low nucleotide diversity, and variable genes are mainly located in a locus known to evolve through homologous recombination. The evolution of AgMNPV is mainly driven by small indels, substitutions, gain and loss of nucleotide stretches or entire coding sequences. These variations may cause relevant phenotypic alterations, which probably affect the infectivity of AgMNPV. This work provides novel information on genomic evolution of the AgMNPV in particular and of baculoviruses in general.
ISSN:1759-6653
1759-6653
DOI:10.1093/gbe/evv231