Microwave gallium-68 radiochemistry for kinetically stable bis(thiosemicarbazone) complexes: structural investigations and cellular uptake under hypoxia† †Electronic supplementary information (ESI) available. CCDC 1001632–1001634. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5dt02537k Click here for additional data file. Click here for additional data file

Hypoxia selectivity of new gallium-68 bis-(thiosemicarbazones) synthesised via microwave heating were investigated. We report the microwave synthesis of several bis(thiosemicarbazones) and the rapid gallium-68 incorporation to give the corresponding metal complexes. These proved kinetically stable u...

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Published in:Dalton transactions : an international journal of inorganic chemistry 2015-11, Vol.45 (1), p.144-155
Main Authors: Alam, Israt S., Arrowsmith, Rory L., Cortezon-Tamarit, Fernando, Twyman, Frazer, Kociok-Köhn, Gabriele, Botchway, Stanley W., Dilworth, Jonathan R., Carroll, Laurence, Aboagye, Eric O., Pascu, Sofia I.
Format: Article
Language:English
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Chemistry
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Summary:Hypoxia selectivity of new gallium-68 bis-(thiosemicarbazones) synthesised via microwave heating were investigated. We report the microwave synthesis of several bis(thiosemicarbazones) and the rapid gallium-68 incorporation to give the corresponding metal complexes. These proved kinetically stable under ‘cold’ and ‘hot’ biological assays and were investigated using laser scanning confocal microscopy, flow cytometry and radioactive cell retention studies under normoxia and hypoxia. 68 Ga complex retention was found to be 34% higher in hypoxic cells than in normoxic cells over 30 min, further increasing to 53% at 120 min. Our data suggests that this class of gallium complexes show hypoxia selectivity suitable for imaging in living cells and in vivo tests by microPET in nude athymic mice showed that they are excreted within 1 h of their administration.
ISSN:1477-9226
1477-9234
DOI:10.1039/c5dt02537k