Multi-functionalized carbon dots as theranostic nanoagent for gene delivery in lung cancer therapy

Theranostics, an integrated therapeutic and diagnostic system, can simultaneously monitor the real-time response of therapy. Different imaging modalities can combine with a variety of therapeutic moieties in theranostic nanoagents. In this study, a multi-functionalized, integrated theranostic nanoag...

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Bibliographic Details
Published in:	Scientific reports 2016-02, Vol.6 (1), p.21170, Article 21170
Main Authors:	Wu, Yu-Fen, Wu, Hsi-Chin, Kuan, Chen-Hsiang, Lin, Chun-Jui, Wang, Li-Wen, Chang, Chien-Wen, Wang, Tzu-Wei
Format:	Article
Language:	eng
Subjects:	Animals Biocompatible Materials Carbon Carbon - chemistry Cell culture Cell Line, Tumor Cyclin B1 Disease Models, Animal Endocytosis Epidermal growth factor receptors Folic acid Folic Acid - chemistry Gene Expression Gene Silencing Gene transfer Gene Transfer Techniques Genes, Reporter Humans Imines - chemistry Luminescence Lung cancer Lung Neoplasms - diagnosis Lung Neoplasms - genetics Lung Neoplasms - therapy Male Materials Testing Mice Molecular Imaging Nanoparticles - chemistry Nanoparticles - ultrastructure Oxidation-Reduction Particle Size Photons Polyethylenes - chemistry Precision medicine RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics siRNA Theranostic Nanomedicine Transgenes
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Summary:	Theranostics, an integrated therapeutic and diagnostic system, can simultaneously monitor the real-time response of therapy. Different imaging modalities can combine with a variety of therapeutic moieties in theranostic nanoagents. In this study, a multi-functionalized, integrated theranostic nanoagent based on folate-conjugated reducible polyethylenimine passivated carbon dots (fc-rPEI-Cdots) is developed and characterized. These nanoagents emit visible blue photoluminescence under 360 nm excitation and can encapsulate multiple siRNAs (EGFR and cyclin B1) followed by releasing them in intracellular reductive environment. In vitro cell culture study demonstrates that fc-rPEI-Cdots is a highly biocompatible material and a good siRNA gene delivery carrier for targeted lung cancer treatment. Moreover, fc-rPEI-Cdots/pooled siRNAs can be selectively accumulated in lung cancer cells through receptor mediated endocytosis, resulting in better gene silencing and anti-cancer effect. Combining bioimaging of carbon dots, stimulus responsive property, gene silencing strategy, and active targeting motif, this multi-functionalized, integrated theranostic nanoagent may provide a useful tool and platform to benefit clinicians adjusting therapeutic strategy and administered drug dosage in real time response by monitoring the effect and tracking the development of carcinomatous tissues in diagnostic and therapeutic aspects.
ISSN:	2045-2322 2045-2322