Revisiting Cardiac Cellular Composition

RATIONALE:Accurate knowledge of the cellular composition of the heart is essential to fully understand the changes that occur during pathogenesis and to devise strategies for tissue engineering and regeneration. OBJECTIVE:To examine the relative frequency of cardiac endothelial cells, hematopoietic-...

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Bibliographic Details
Published in:Circulation research 2016-02, Vol.118 (3), p.400-409
Main Authors: Pinto, Alexander R, Ilinykh, Alexei, Ivey, Malina J, Kuwabara, Jill T, D’Antoni, Michelle L, Debuque, Ryan, Chandran, Anjana, Wang, Lina, Arora, Komal, Rosenthal, Nadia A, Tallquist, Michelle D
Format: Article
Language:English
Subjects:
Adult
Animals
Biomarkers - metabolism
Cell Count
Cell Differentiation
Cell Lineage
Cell Separation - methods
Endothelial Cells - metabolism
Female
Fibroblasts - metabolism
Flow Cytometry
Gene Expression Regulation
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Heart
Hematopoietic Stem Cells - metabolism
Humans
Immunohistochemistry
Male
Mice, Inbred C57BL
Mice, Transgenic
Middle Aged
Phenotype
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Summary:RATIONALE:Accurate knowledge of the cellular composition of the heart is essential to fully understand the changes that occur during pathogenesis and to devise strategies for tissue engineering and regeneration. OBJECTIVE:To examine the relative frequency of cardiac endothelial cells, hematopoietic-derived cells, and fibroblasts in the mouse and human heart. METHODS AND RESULTS:Using a combination of genetic tools and cellular markers, we examined the occurrence of the most prominent cell types in the adult mouse heart. Immunohistochemistry revealed that endothelial cells constitute >60%, hematopoietic-derived cells 5% to 10%, and fibroblasts
ISSN:0009-7330
1524-4571
DOI:10.1161/CIRCRESAHA.115.307778