Microphotocoagulation: Selective Effects of Repetitive Short Laser Pulses

Repetitive exposure to short laser pulses is shown to cause selective damage to absorbing structures (cells, organelles, or enzymes) with pulse energies below the threshold energy for single-pulse damage. Directly adjacent structures are spared in vivo. Additivity of (presumably nonphotochemical) su...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-09, Vol.90 (18), p.8643-8647
Main Authors: Roider, Johann, Hillenkamp, Franz, Flotte, Thomas, Birngruber, Reginald
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cellular biology
Effects of various physical factors on living matter (vibrations, electric field, ultrasound, sound...)
Flood damage
Fluorescein Angiography
Fundamental and applied biological sciences. Psychology
Irradiation
Laser damage
Lasers
Lesions
Light Coagulation - adverse effects
Light Coagulation - methods
Melanin
Microscopy, Electron
Microsurgery - adverse effects
Microsurgery - methods
Molecules
Photoreceptors
Probability
Rabbits
Retina
Retina - pathology
Retina - radiation effects
Retina - ultrastructure
Temperature gradients
Time Factors
Tissues, organs and organisms biophysics
Yield point
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Repetitive exposure to short laser pulses is shown to cause selective damage to absorbing structures (cells, organelles, or enzymes) with pulse energies below the threshold energy for single-pulse damage. Directly adjacent structures are spared in vivo. Additivity of (presumably nonphotochemical) subthreshold effects is demonstrated. Selective damage to the retinal pigment epithelium with sparing of the neural retina is shown (514 nm, 5 μ s, 1-500 pulses at 500 Hz, 2- to 10-μ J pulse energy). A melanin granule model has been developed and applied to the experimental situation. Histological results as well as the basic mechanism for these effects are discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.18.8643