Fangchinoline inhibits the proliferation of SPC-A-1 lung cancer cells by blocking cell cycle progression

Fangchinoline (Fan) is a bioactive compound isolated from the Chinese herb Stephania tetrandra S. Moore (Fen Fang Ji). The aim of the present study was to investigate the effect of Fan on the proliferation of SPC-A-1 lung cancer cells, and to define the associated molecular mechanisms. Following tre...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2016-02, Vol.11 (2), p.613-618
Main Authors: LUO, XUE, PENG, JIAN-MING, SU, LAN-DI, WANG, DONG-YAN, YU, YOU-JIANG
Format: Article
Language:English
Subjects:
Antimitotic agents
Antineoplastic agents
Cancer
Cancer therapies
Cell cycle
Cell growth
Complications and side effects
cyclin D1
Cyclin-dependent kinases
Dosage and administration
Drug therapy
fangchinoline
Kinases
Laboratories
Leukemia
Lung cancer
Phase transitions
Phosphorylation
Proteins
Retina
Retinoblastoma
Studies
Tumors
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Summary:Fangchinoline (Fan) is a bioactive compound isolated from the Chinese herb Stephania tetrandra S. Moore (Fen Fang Ji). The aim of the present study was to investigate the effect of Fan on the proliferation of SPC-A-1 lung cancer cells, and to define the associated molecular mechanisms. Following treatment with Fan, Cell Counting Kit-8, phase contrast imaging and Giemsa staining assays were used to detect cell viability; flow cytometry was performed to analyze the cell cycle distribution; and reverse transcription-quantitative polymerase chain reaction and western blot assays were used to investigate changes in the expression levels of cell cycle-associated genes and proteins. In the present study, treatment with Fan markedly inhibited the proliferation of SPC-A-1 lung cancer cells and significantly increased the percentage of cells in the G0/G1 phase of the cell cycle in a dose-dependent manner (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2015.2915